Luo Wen, Gudipati Murali, Jung Kevin, Chen Mao, Marschke Keith B
Ligand Pharmaceuticals INC, San Diego, CA 92121, USA.
J Integr Bioinform. 2009 Aug 23;6(1):108. doi: 10.2390/biecoll-jib-2009-108.
Despite the large number of software tools developed to address different areas of microarray data analysis, very few offer an all-in-one solution with little learning curve. For microarray core labs, there are even fewer software packages available to help with their routine but critical tasks, such as data quality control (QC) and inventory management. We have developed a simple-to-use web portal to allow bench biologists to analyze and query complicated microarray data and related biological pathways without prior training. Both experiment-based and gene-based analysis can be easily performed, even for the first-time user, through the intuitive multi-layer design and interactive graphic links. While being friendly to inexperienced users, most parameters in Goober can be easily adjusted via drop-down menus to allow advanced users to tailor their needs and perform more complicated analysis. Moreover, we have integrated graphic pathway analysis into the website to help users examine microarray data within the relevant biological content. Goober also contains features that cover most of the common tasks in microarray core labs, such as real time array QC, data loading, array usage and inventory tracking. Overall, Goober is a complete microarray solution to help biologists instantly discover valuable information from a microarray experiment and enhance the quality and productivity of microarray core labs. The whole package is freely available at http://sourceforge.net/projects/goober. A demo web server is available at http://www.goober-array.org.
尽管已经开发了大量用于处理微阵列数据分析不同领域的软件工具，但很少有工具能提供几乎没有学习曲线的一体化解决方案。对于微阵列核心实验室来说，可用于帮助其完成日常但关键任务（如数据质量控制（QC）和库存管理）的软件包更少。我们开发了一个易于使用的门户网站，使实验台生物学家无需事先培训就能分析和查询复杂的微阵列数据及相关生物途径。通过直观的多层设计和交互式图形链接，即使是首次使用的用户也能轻松进行基于实验和基于基因的分析。虽然对没有经验的用户很友好，但Goober中的大多数参数都可以通过下拉菜单轻松调整，以便高级用户根据自己的需求进行定制并执行更复杂的分析。此外，我们还将图形化途径分析集成到网站中，以帮助用户在相关生物内容中检查微阵列数据。Goober还包含了涵盖微阵列核心实验室大多数常见任务的功能，如实时阵列QC、数据加载、阵列使用和库存跟踪。总体而言，Goober是一个完整的微阵列解决方案，可帮助生物学家立即从微阵列实验中发现有价值的信息，并提高微阵列核心实验室的质量和生产力。整个软件包可在http://sourceforge.net/projects/goober上免费获取。演示网络服务器可在http://www.goober-array.org上访问。
