Experimental and Computational Laboratory for the Analysis of Turbulence, Division of Engineering, King's College London, Strand, London, UK.
Clin Hemorheol Microcirc. 2010;44(1):43-54. doi: 10.3233/CH-2009-1251.
In the present study electro-rheology (Contraves LS30 viscometer-based system) and optical shearing microscopy (Lincam CSS450 system and image analysis) techniques have been utilized in order to provide quantitative data on the behaviour of the microstructural properties of whole normal human blood at non-steady flow conditions. The objective of this work is to contribute towards a better understanding of red blood cell aggregation at flow conditions similar to that occurring in a circulatory system and to aid the interpretation and validation of electro-rheological data through a quantitative comparison with data acquired with optical shearing microscopy. Electro-rheology is a promising technique that has been used to provide bulk fluid properties, showing potential for basic research and diagnostic purposes, whereas optical shearing techniques offer a direct assessment of blood microstructure at a cellular level. However, little information exists in the literature regarding the relationships between electro-rheological measurements and blood microstructural characteristics. The results showed that the different non-steady flow conditions affect differently the dynamics of aggregation varying from a parabolic-decrease to an inverted S-shape curve with time. For a wide range of the non-steady flows results obtained with the two different techniques agree to a difference between 1.2 and 12%.
在本研究中，采用电流变学（基于 Contraves LS30 粘度计的系统）和光剪切显微镜（Lincam CSS450 系统和图像分析）技术，提供全血在非稳态流动条件下的微观结构特性行为的定量数据。这项工作的目的是有助于更好地理解在类似于循环系统中发生的流动条件下红细胞的聚集，并通过与光剪切显微镜获得的数据进行定量比较，为电流变学数据的解释和验证提供帮助。电流变学是一种很有前途的技术，已被用于提供整体流体性质，显示出在基础研究和诊断方面的潜力，而光剪切技术则可以直接评估细胞水平的血液微观结构。然而，文献中关于电流变学测量与血液微观结构特征之间的关系的信息很少。结果表明，不同的非稳态流动条件以不同的方式影响聚集的动力学，随时间呈现抛物线减少到倒 S 形曲线的变化。对于广泛的非稳态流动，两种不同技术的结果之间的差异在 1.2 到 12%之间。
