强效生长刺激因子碱性成纤维细胞生长因子（bFGF）和生长调节因子转化生长因子-β（TGF-β）对体外培养的巩膜软骨细胞和巩膜成纤维细胞的影响。

Influence of bFGF as a potent growth stimulator and TGF-beta as a growth regulator on scleral chondrocytes and scleral fibroblasts in vitro.

作者信息

Seko Y, Tanaka Y, Tokoro T

机构信息

Department of Ophtalmology, Tokyo Medical and Dental University School of Medicine, Japan.

出版信息

Ophthalmic Res. 1995;27(3):144-52. doi: 10.1159/000267651.

DOI:10.1159/000267651

PMID:8538991

Abstract

We investigated the proliferation of scleral cells in response to several growth factors in vitro to elucidate the mechanism of scleral growth in visual deprivation myopia. Scleral chondrocytes and scleral fibroblasts were cultured separately in 24-well culture dishes. The number of plated cells was counted after the addition of basic fibroblast growth factor (bFGF), transforming growth factor alpha (TGF-alpha), TGF-beta, insulin-like growth factor I (IGF-I), IGF-II, platelet-derived growth factor AA (PDGF-AA), PDGF-AB and PDGF-BB. All the growth factors studied, except for PDGF-AA, stimulated the proliferation of both scleral chondrocytes and scleral fibroblasts. bFGF showed the highest effect. TGF-beta caused morphologic changes in both scleral chondrocytes and scleral fibroblasts. Various growth factors stimulated the proliferation of scleral chondrocytes and scleral fibroblasts in a similar manner. bFGF was a potent growth stimulator and TGF-beta was suggested to be a growth regulator on scleral chondrocytes and scleral fibroblasts.

摘要

我们在体外研究了巩膜细胞对几种生长因子的增殖反应，以阐明形觉剥夺性近视中巩膜生长的机制。将巩膜软骨细胞和巩膜成纤维细胞分别培养于24孔培养板中。加入碱性成纤维细胞生长因子（bFGF）、转化生长因子α（TGF-α）、TGF-β、胰岛素样生长因子I（IGF-I）、IGF-II、血小板衍生生长因子AA（PDGF-AA）、PDGF-AB和PDGF-BB后，对接种的细胞进行计数。除PDGF-AA外，所有研究的生长因子均刺激巩膜软骨细胞和巩膜成纤维细胞的增殖。bFGF的作用最为显著。TGF-β引起巩膜软骨细胞和巩膜成纤维细胞的形态学改变。各种生长因子以相似的方式刺激巩膜软骨细胞和巩膜成纤维细胞的增殖。bFGF是一种有效的生长刺激剂，TGF-β被认为是巩膜软骨细胞和巩膜成纤维细胞的生长调节剂。