3D Printing and Biofabrication Group, Institute of Materials Science and Technology, TU Wien, Vienna, Austria.
Austrian Cluster for Tissue Regeneration, Vienna, Austria.
Hernia. 2020 Dec;24(6):1233-1243. doi: 10.1007/s10029-020-02135-4. Epub 2020 Feb 24.
The interest in non-manipulated cells originating from adipose tissue has raised tremendously in the field of tissue engineering and regenerative medicine. The resulting stromal vascular fraction (SVF) cells have been successfully used in numerous clinical applications. The aim of this experimental work is, first to combine a macroporous synthetic mesh with SVF isolated using a mechanical disruption process, and to assess the effect of those cells on the early healing phase of hernia.
Human SVF cells combined with fibrin were used to coat commercial titanized polypropylene meshes. In vitro, viability and growth of the SVF cells were assessed using live/dead staining and scanning electron microscopy. The influence of SVF cells on abdominal wall hernia healing was conducted on immunodeficient rats, with a focus on short-term vascularization and fibrogenesis.
Macroporous meshes were easily coated with SVF using a fibrin gel as temporary carrier. The in vitro experiments showed that the whole process including the isolation of human SVF cells and their coating on PP meshes did not impact on the SVF cells' viability and on their capacity to attach and to proliferate. In vivo, the SVF cells were well tolerated by the animals, and coating mesh with SVF resulted in a decrease degree of vascularity compared to control group at day 21.
The utilization of SVF-coated mesh influences the level of angiogenesis during the early onset of tissue healing. Further long-term animal experiments are needed to confirm that this effect correlates with a more robust mesh integration compared to non-SVF-coated mesh.
在组织工程和再生医学领域，人们对源自脂肪组织的未处理细胞产生了浓厚的兴趣。由此产生的基质血管部分 (SVF) 细胞已成功应用于许多临床应用中。本实验旨在将多孔合成网与使用机械破坏过程分离的 SVF 结合，并评估这些细胞对疝早期愈合阶段的影响。
使用纤维蛋白将人 SVF 细胞与纤维蛋白结合，以涂覆商业钛化聚丙烯网。在体外，使用活/死染色和扫描电子显微镜评估 SVF 细胞的活力和生长。通过免疫缺陷大鼠研究 SVF 细胞对腹壁疝愈合的影响，重点关注短期血管生成和纤维发生。
多孔网很容易用纤维蛋白凝胶作为临时载体涂覆 SVF。体外实验表明，包括分离人 SVF 细胞及其涂覆在 PP 网在内的整个过程均不会影响 SVF 细胞的活力及其附着和增殖的能力。在体内，SVF 细胞被动物很好地耐受，与对照组相比，SVF 涂层网在第 21 天的血管生成程度降低。
SVF 涂层网的使用会影响组织愈合早期的血管生成水平。需要进一步的长期动物实验来证实这种效果与非 SVF 涂层网相比，与更稳健的网整合相关。
