Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, China.
Curr Microbiol. 2010 Sep;61(3):190-6. doi: 10.1007/s00284-010-9595-4. Epub 2010 Feb 5.
Orf94 (Bm94) of Bombyx mori nucleopolyhedrovirus (BmNPV) potentially encodes 424-amino acids with a predicted molecular weight of 49.4 kDa, but its function remains unknown. Blast search results revealed that Bm94 homologues exist in 10 completely sequenced Lepidopteron NPVs with identities ranging from 95 to 37%. Results of our recent study showed that Bm94 was transcribed from 12 to 72 h and the corresponding protein was detected from 24 to 72 h post-infection. Furthermore, Western blot analysis revealed that Bm94 was present in occlusion-derived virus (ODV) and in total protein from BmNPV-infected BmN cells, but not in budded virus. Immunofluorescence analysis revealed that the protein located primarily in the cytoplasm and was also present in the nucleus in the later infection. In conclusion, these results together indicated that Bm94 was a late gene, which distributed both in the cytoplasm and in the nucleus, and was identified to be a component of BmNPV ODV.
家蚕核型多角体病毒的 Orf94（Bm94）可能编码 424 个氨基酸，预测分子量为 49.4 kDa，但功能未知。Blast 搜索结果表明，Bm94 同源物存在于 10 种完全测序的鳞翅目杆状病毒中，同一性范围为 95%至 37%。我们最近的研究结果表明，Bm94 在感染后 12 至 72 小时转录，相应的蛋白质在 24 至 72 小时检测到。此外，Western blot 分析表明，Bm94 存在于出芽病毒（ODV）和 BmNPV 感染的 BmN 细胞的总蛋白中，但不存在于包埋病毒中。免疫荧光分析表明，该蛋白主要位于细胞质中，在后期感染时也存在于细胞核中。综上所述，这些结果表明 Bm94 是一个晚期基因，分布在细胞质和细胞核中，并被鉴定为 BmNPV ODV 的组成部分。
