Institute of Life Sciences, Jiangsu University, Zhenjiang, People's Republic of China.
Curr Microbiol. 2010 Nov;61(5):451-7. doi: 10.1007/s00284-010-9637-y. Epub 2010 Apr 6.
Bombyx mori nucleopolyhedrovirus (BmNPV) ORF109 (Bm109) is a gene present in many lepidopteran NPVs, but its function is unknown. In this study, Bm109 was characterized. The transcript of Bm109 was detected at 12-72 h postinfection (p.i.). Polyclonal antiserum raised to a His-BM109 fusion protein recognized BM109 in infected cell lysates from 24 to 72 h p.i., suggesting that Bm109 is a late gene. Localization of the BM109 in BV and ODV by western analyses demonstrated that BM109 was proteins of ODV.
家蚕核型多角体病毒（BmNPV）ORF109（Bm109）是许多鳞翅目 NPV 中存在的一个基因，但它的功能尚不清楚。本研究对 Bm109 进行了表征。在感染后 12-72 小时检测到 Bm109 的转录本。针对 His-BM109 融合蛋白的多克隆抗血清在感染后 24 至 72 小时的细胞裂解物中识别出 BM109，表明 Bm109 是一个晚期基因。通过 Western 分析对 BV 和 ODV 中的 BM109 进行定位，证明 BM109 是 ODV 的蛋白质。
