Plant Bioengineering Laboratory, Northeast Agricultural University, Harbin, China.
Planta. 2010 Apr;231(5):991-1001. doi: 10.1007/s00425-010-1104-2. Epub 2010 Feb 5.
Salt and alkali stress are two of the main environmental factors limiting rice production. Thus, understanding the mechanisms of salinity and alkali stress tolerance is necessary to modify rice to increase its resistance to salinity and alkaline stress. MicroRNAs (miRNAs) are approximately 21-nucleotide RNAs that are ubiquitous regulators of gene expression in eukaryotic organisms. In plants, miRNAs constitute one of five classes of small RNAs that function primarily as negative regulators for gene expression at the posttranscriptional level. Several plant miRNAs, such as miR396, play vital roles in plant growth, development and resistance to stresses. In this study, we identified osa-MIR396c, which shows dramatic transcript change under salt and alkali stress conditions in Oryza sativa. We designed an experiment to detect miRNA-target interaction and demonstrated that several transcription factors related to growth, development, and stress tolerance are targeted by osa-MIR396c. Transgenic rice and Arabidopsis thaliana plants constitutively over-expressing osa-MIR396c showed reduced salt and alkali stress tolerance compared to that of wild-type plants. Overall, this study further established a link between salt and alkali stress and osa-MIR396c in rice.
盐和碱胁迫是限制水稻生产的两个主要环境因素。因此，了解耐盐和耐碱胁迫的机制对于改良水稻以提高其耐盐碱性是必要的。 microRNAs (miRNAs) 是大约 21 个核苷酸的 RNA，是真核生物中基因表达的普遍调节因子。在植物中，miRNAs 构成了五类小 RNA 中的一类，主要作为转录后水平基因表达的负调节剂发挥作用。一些植物 miRNAs，如 miR396，在植物生长、发育和抗逆性中发挥着重要作用。在本研究中，我们鉴定了 Oryza sativa 中盐和碱胁迫条件下转录变化显著的 osa-MIR396c。我们设计了一个实验来检测 miRNA 靶标相互作用，并证明了几个与生长、发育和耐逆性相关的转录因子是 osa-MIR396c 的靶标。过表达 osa-MIR396c 的转基因水稻和拟南芥植物与野生型植物相比，耐盐碱性降低。总的来说，这项研究进一步确立了水稻中盐和碱胁迫与 osa-MIR396c 之间的联系。
