Sheng Wan, Zhang Guogen, Zhai Laiyuan, Xu Jianlong
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Rice Research Institute, Shenyang Agricultural University, Shenyang, 110866, China.
Sci Rep. 2024 Dec 3;14(1):30063. doi: 10.1038/s41598-024-79273-7.
Alkali stress is one of the most serious abiotic stresses limiting crop yield and it has become an increasingly serious global problem in recent years. Alkalinity tolerance (AT) at the seedling stage is one of the determinant factors for establishment of rice population under alkaline stress condition. Here, we evaluated and measured seven traits related to AT of 528 diverse rice accessions at the seedling stage. Xian accessions were generally more alkali-tolerant than Geng accessions. GJ-tmp accessions showed the most alkali tolerance in the Geng subgroups and XI-1B accessions had the weakest alkali tolerance in the Xian subgroups. A total of 121 QTLs were identified for AT by genome-wide association study (GWAS), and five important candidate genes, LOC_Os01g19800, LOC_Os01g20160, LOC_Os01g52500, LOC_Os01g67370 and LOC_Os03g03900, were selected by gene function annotation, haplotype analysis, and qRT-PCR. Pyramiding of multiple AT advanced candidate genes is a favorable strategy for improving AT of rice varieties. Our study has screened alkali-tolerant germplasm resources and provided valuable genetic information for alkali-tolerant rice breeding.
碱胁迫是限制作物产量的最严重非生物胁迫之一，近年来已成为一个日益严重的全球性问题。苗期耐碱性（AT）是碱性胁迫条件下水稻群体建立的决定性因素之一。在此，我们评估并测定了528份不同水稻品种在苗期与AT相关的7个性状。籼稻品种通常比粳稻品种更耐碱。GJ-tmp品种在粳稻亚群中表现出最强的耐碱性，而XI-1B品种在籼稻亚群中耐碱性最弱。通过全基因组关联研究（GWAS）共鉴定出121个与AT相关的数量性状基因座（QTL），并通过基因功能注释、单倍型分析和qRT-PCR筛选出5个重要的候选基因，即LOC_Os01g19800、LOC_Os01g20160、LOC_Os01g52500、LOC_Os01g67370和LOC_Os03g03900。聚合多个AT高级候选基因是提高水稻品种AT的有利策略。我们的研究筛选了耐碱种质资源，为耐碱水稻育种提供了有价值的遗传信息。
