嗜热自养甲烷杆菌DeltaH在缺氢条件下无法生长的突变体的分离与鉴定。
Isolation and characterization of Methanobacterium thermoautotrophicum DeltaH mutants unable to grow under hydrogen-deprived conditions.
作者信息
Pennings J L, Keltjens J T, Vogels G D
机构信息
Department of Microbiology, University of Nijmegen, Nijmegen, The Netherlands.
出版信息
J Bacteriol. 1998 May;180(10):2676-81. doi: 10.1128/JB.180.10.2676-2681.1998.
Abstract
By using random mutagenesis and enrichment by chemostat culturing, we have developed mutants of Methanobacterium thermoautotrophicum that were unable to grow under hydrogen-deprived conditions. Physiological characterization showed that these mutants had poorer growth rates and growth yields than the wild-type strain. The mRNA levels of several key enzymes were lower than those in the wild-type strain. A fed-batch study showed that the expression levels were related to the hydrogen supply. In one mutant strain, expression of both methyl coenzyme M reductase isoenzyme I and coenzyme F420-dependent 5,10-methylenetetrahydromethanopterin dehydrogenase was impaired. The strain was also unable to form factor F390, lending support to the hypothesis that the factor functions in regulation of methanogenesis in response to changes in the availability of hydrogen.
摘要
通过随机诱变和恒化器培养富集，我们培育出了嗜热自养甲烷杆菌的突变体，这些突变体在氢气缺乏的条件下无法生长。生理学特性表明，这些突变体的生长速率和生长产量比野生型菌株低。几种关键酶的mRNA水平低于野生型菌株。补料分批培养研究表明，这些酶的表达水平与氢气供应有关。在一个突变菌株中，甲基辅酶M还原酶同工酶I和辅酶F420依赖的5,10-亚甲基四氢甲蝶呤脱氢酶的表达均受到损害。该菌株也无法形成因子F390，这支持了该因子在响应氢气可用性变化时调节甲烷生成的假说。
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