Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
J Am Chem Soc. 2010 Mar 3;132(8):2795-801. doi: 10.1021/ja910090v.
Reactive oxygen species (ROS) operate as signaling molecules under various physiological conditions, and overproduction of ROS is involved in the pathogenesis of many diseases. Therefore, fluorescent probes for visualizing ROS are promising tools with which to uncover the molecular mechanisms of physiological and pathological processes and might also be useful for diagnosis. Here we describe a novel fluorescence probe, FOSCY-1, operating in the physiologically favorable near-infrared region. The probe consists of two differentially ROS-reactive cyanine dyes connected by a linker; reaction of the more susceptible dye with ROS releases intramolecular fluorescence quenching of the less susceptible dye. We successfully applied this probe to detect ROS produced by HL60 cells and porcine neutrophils and for imaging oxidative stress in a mouse model of peritonitis.
活性氧（ROS）在各种生理条件下作为信号分子发挥作用，ROS 的过度产生与许多疾病的发病机制有关。因此，用于可视化 ROS 的荧光探针是揭示生理和病理过程分子机制的有前途的工具，并且对于诊断也可能是有用的。在这里，我们描述了一种新型的荧光探针 FOSCY-1，它在生理上有利的近红外区域运行。该探针由通过连接体连接的两个对 ROS 具有不同反应性的氰基染料组成；更易受影响的染料与 ROS 的反应释放出对较少易受影响的染料的分子内荧光猝灭。我们成功地将该探针应用于检测 HL60 细胞和猪中性粒细胞产生的 ROS，并用于对腹膜炎小鼠模型中的氧化应激进行成像。
