Department of Pathology, All India Institute of Medical Sciences (AIIMS), Ansari Nagar, New Delhi, India.
Immunopharmacol Immunotoxicol. 2010 Sep;32(3):523-7. doi: 10.3109/08923970903581540.
The aim of the present investigation was to evaluate the efficacy of Withania somnifera on tricarboxylic acid (TCA) cycle enzymes and electron transport chain in azoxymethane-induced experimental colon cancer in mice. Azoxymethane at the dose of 15 mg/kg body weight was induced intraperitoneally once in a week for 28 days. The progression in colon tumor development was correlated with the appearance of the histological biomarker and aberrant crypt foci (ACF). Azoxymethane-induced colon cancer animals were treated with 400 mg/kg body weight of W. somnifera once in a week orally for 28 days. After the experimental period, the animals were killed and analyzed for TCA cycle key enzymes, such as isocitrate dehydrogenase (ICDH), succinate dehydrogenase (SDH), malate dehydrogenase (MDH), and alpha-keto glutarate dehydrogenase (alpha-KGDH). The modulating effect of W. somnifera on TCA cycle key enzymes and electron transport chain complexes were investigated against colon cancer induced by azoxymethane in Swiss albino mice. Decreased activities of TCA cycle key enzymes such as ICDH, SDH, MDH, and alpha-KGDH in colon cancer bearing animals were observed. W. somnifera administration normalized these enzyme levels in azoxymethane-induced experimental mice. These results suggested that W. somnifera is the promising chemotherapeutic agent for the treatment of colon cancer.
本研究旨在评估睡茄（Withania somnifera）对三羧酸（TCA）循环酶和氧化磷酸化电子传递链在氧化偶氮甲烷诱导的实验性结肠癌细胞中的作用。每周一次腹腔内注射 15mg/kg 体重的氧化偶氮甲烷，共 28 天。结肠肿瘤进展与组织学生物标志物和异常隐窝病灶（ACF）的出现相关。用 400mg/kg 体重的睡茄每周一次灌胃处理氧化偶氮甲烷诱导的结肠癌动物，共 28 天。实验结束后，处死动物，分析 TCA 循环关键酶，如异柠檬酸脱氢酶（ICDH）、琥珀酸脱氢酶（SDH）、苹果酸脱氢酶（MDH）和α-酮戊二酸脱氢酶（α-KGDH）。研究睡茄对瑞士白化小鼠氧化偶氮甲烷诱导的结肠癌的 TCA 循环关键酶和氧化磷酸化电子传递链复合物的调节作用。在患有结肠癌的动物中观察到 TCA 循环关键酶如 ICDH、SDH、MDH 和α-KGDH 的活性降低。睡茄给药可使这些酶在氧化偶氮甲烷诱导的实验性小鼠中恢复正常水平。这些结果表明，睡茄是治疗结肠癌的有前途的化疗药物。
