Su Wen-Jin, Gan Lu, Wang Hai-Jiao, Zhou Ting, Weng Shao-Fan, Liu Fang-Chao, Chen Wei-Hong
MOE Key Lab of Environmental and Health and Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2009 Oct;27(10):579-83.
To assess biological response and health adverse effects of industrial dusts from pottery factories and tungsten mines on alveolar macrophages (AM) in vitro.
AM acquired from bronchoalveolar lavage of guinea pigs were used as the target cells. AM were then co-cultured with respirable dust particles (15, 30, 60 and 120 μg/10⁶) from pottery factories and tungsten mines. LDH activity, cell viability, the release of ROS and TNF-α were determined to assess the biological responses of the dusts. China Standard Quartz was used as control.
Dose- response relationships between the dust concentrations and the enhancement of LDH activity, the release of ROS and TNF-α were found in both dusts from pottery factories and tungsten mines. The cell viability decreased when the dusts' concentrations increased. Differences of biological response were observed in the dust particles from different mines or factories. Compared with the pottery dusts, higher LDH activity and the release of TNF-α induced by tungsten dust were observed. In the 120 μg/10⁶ group, the TNF-α induced by tungsten dust, pottery dusts and China Standard Quartz was (5.2 +/- 2.0) ng/ml, (3.3 +/- 1.6) ng/ml and (2.8 +/- 0.5) ng/ml respectively. However, the impact on the cell viability induced by pottery dust was higher than that by tungsten mine.
Industrial dusts from various sources could induce different biological effects. The results of the biological effects of dusts in laboratory tests may be of potential use to provide base data for their adverse effects evaluation.
评估陶瓷厂和钨矿工业粉尘对体外肺泡巨噬细胞（AM）的生物学反应和健康不良影响。
将从豚鼠支气管肺泡灌洗中获取的AM用作靶细胞。然后将AM与陶瓷厂和钨矿的可吸入粉尘颗粒（15、30、60和120μg/10⁶）共培养。测定乳酸脱氢酶（LDH）活性、细胞活力、活性氧（ROS）和肿瘤坏死因子-α（TNF-α）的释放，以评估粉尘的生物学反应。以中国标准石英作为对照。
在陶瓷厂和钨矿的粉尘中均发现粉尘浓度与LDH活性增强、ROS和TNF-α释放之间存在剂量反应关系。随着粉尘浓度增加，细胞活力下降。在不同矿山或工厂的粉尘颗粒中观察到生物学反应的差异。与陶瓷粉尘相比，钨粉尘诱导的LDH活性和TNF-α释放更高。在120μg/10⁶组中，钨粉尘、陶瓷粉尘和中国标准石英诱导的TNF-α分别为（5.2±2.0）ng/ml、（3.3±1.6）ng/ml和（2.8±0.5）ng/ml。然而，陶瓷粉尘对细胞活力的影响高于钨矿粉尘。
不同来源的工业粉尘可诱导不同的生物学效应。粉尘在实验室测试中的生物学效应结果可能有助于为其不良影响评估提供基础数据。
