Song Wei-qing, Li Hua-hui, Chen Hua-bo, Yuan Jiang-shui, Yin Xiao-jie
Department of Laboratory Medicine, Qingdao Municipal Hospital, China.
Zhonghua Yi Xue Za Zhi. 2009 Nov 24;89(43):3038-42.
To investigate two single nucleotide polymorphism sites of IRF5 and TLR-9 and to detect their relationship with SLE (systemic lupus erythematosus) in a Han population from Shandong province.
The polymorphisms of rs2004640 G/T, rs10954213 G/A in IRF-5 and rs187084C/T, rs352139A/G in TLR-9 were detected with PCR-RFLP in 92 cases of SLE and 88 healthy controls. The genotype and allele frequencies were calculated and analyzed.
(1) The genotype frequencies of GG, GT and TT in rs2004640 site in SLE were 0.198, 0.521 and 0.281 respectively. The difference was significant between SLE and controls (chi(2) = 8.73, P < 0.05); the genotype frequencies of GG, GA and AA at rs109542130 site in SLE were 0.318, 0.409 and 0.273 respectively. The difference was significant between SLE and controls (chi(2) = 6.36, P < 0.05). (2) The genotype frequencies of CC, CT and TT at rs187084 site in SLE were 0.185, 0.413 and 0.402 respectively. There was no difference between SLE and controls (chi(2) = 2.99, P > 0.05); the genotype frequencies of AA, AG and GG at rs352139 site in SLE were 0.228, 0.533 and 0.239 respectively. There was no difference between SLE and controls (chi(2) = 4.54, P > 0.05).
The polymorphism of rs2004640 and rs10954213 in IRF5 may be associated with SLE in the population of Han nationality from Shandong province. However, the polymorphism of rs187084 and rs352139 in TLR-9 is not associated with SLE.
研究干扰素调节因子5（IRF5）和Toll样受体9（TLR - 9）的两个单核苷酸多态性位点，并检测其与山东省汉族人群系统性红斑狼疮（SLE）的关系。
采用聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）技术检测92例SLE患者和88例健康对照者中IRF - 5基因的rs2004640 G/T、rs10954213 G/A以及TLR - 9基因的rs187084C/T、rs352139A/G多态性。计算并分析基因型和等位基因频率。
（1）SLE患者rs2004640位点GG、GT和TT基因型频率分别为0.198、0.521和0.281。SLE组与对照组差异有统计学意义（χ² = 8.73，P < 0.05）；SLE患者rs109542130位点GG、GA和AA基因型频率分别为0.318、0.409和0.273。SLE组与对照组差异有统计学意义（χ² = 6.36，P < 0.05）。（2）SLE患者rs187084位点CC、CT和TT基因型频率分别为0.185、0.413和0.402。SLE组与对照组差异无统计学意义（χ² = 2.99，P > 0.05）；SLE患者rs352139位点AA、AG和GG基因型频率分别为0.228、0.533和0.239。SLE组与对照组差异无统计学意义（χ² = 4.54，P > 0.05）。
IRF5基因的rs2004640和rs10954213多态性可能与山东省汉族人群的SLE发病相关。然而，TLR - 9基因的rs187084和rs352139多态性与SLE发病无关。
