Fakultät für Physik, Universität Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany.
J Biotechnol. 2010 Sep 15;149(4):267-71. doi: 10.1016/j.jbiotec.2010.01.018. Epub 2010 Feb 4.
Fluorescent labeling of self-assembled monolayers (SAMs) has a great potential for chemical and biotechnological sensing. However, its use is limited by the quenching of the fluorescence in the proximity of the conducting substrates. We show that this quenching can be overcome by the labeling of a cross-linked aromatic SAM (nanosheet) and its subsequent transfer onto a non-conducting substrate. We demonstrate the successful labeling of nanosheets with a fluorophore (tetramethylrhodamine) and its subsequent transfer to oxidized silicon, where they are detected by optical as well as fluorescence microscopy. Fluorescently labeled freestanding nanosheets, i.e. nanomembranes were obtained by a similar transfer of the nanosheets to TEM grids.
自组装单分子层（SAMs）的荧光标记在化学和生物技术传感方面具有巨大的潜力。然而，其应用受到在导电衬底附近荧光猝灭的限制。我们表明，通过对交联的芳族 SAM（纳米片）进行标记，并将其随后转移到非导电衬底上，可以克服这种猝灭。我们证明了用荧光团（四甲基罗丹明）成功标记纳米片，并将其随后转移到氧化硅上，在那里可以通过光学显微镜和荧光显微镜检测到它们。通过类似的方法将纳米片转移到 TEM 网格上，得到了荧光标记的独立纳米片，即纳米膜。
