Department of Biomedical Science, CHA University, Kyonggi, Seongnam 463-836, Republic of Korea.
Food Chem Toxicol. 2010 Apr;48(4):1101-4. doi: 10.1016/j.fct.2010.01.032. Epub 2010 Feb 4.
The sulfated seaweed extract, fucoidan, has anticoagulant, antithrombotic, and antiviral activities. Despite extensive work on the biological activities of fucoidan, detailed studies on the genotoxicity of fucoidan from Sporophyll of Undaria pinnatifida sources have not been tested before. The objective of this study was to investigate the genotoxicity effects of fucoidan extracted from Sporophyll of U. pinnatifida using a test battery of three different methods. In a reverse mutation assay using four Salmonella typhimurium strains and Escherichia coli, fucoidan did not increase the number of revertant colonies in any tester strain regardless of metabolic activation by S9 mix, and did not cause chromosomal aberration in short tests with S9 mix or in the continuous (24 h) test. A bone marrow micronucleus test in ICR mice dosed by oral gavage at doses up to 2000 mg/kg bw/day showed no significant or dose-dependent increases in the frequency of micronucleated polychromatic erythrocytes (MNPCE), and the high dose suppressed the ratio of polychromatic erythrocytes (PCE) to total erythrocytes. We conclude that fucoidan presents no significant genotoxic concern under the anticipated conditions of use.
硫酸化海藻提取物岩藻聚糖具有抗凝、抗血栓形成和抗病毒活性。尽管已经对岩藻聚糖的生物活性进行了广泛的研究，但之前尚未对来自裙带菜孢子叶的岩藻聚糖的详细遗传毒性进行测试。本研究的目的是使用三种不同方法的测试组合来研究岩藻聚糖的遗传毒性作用。在使用 4 种鼠伤寒沙门氏菌和大肠杆菌的回复突变试验中，无论是否通过 S9 混合物进行代谢激活，岩藻聚糖都不会增加任何测试菌株的回复菌落数量，并且不会在 S9 混合物的短期试验或连续（24 小时）试验中引起染色体畸变。在 ICR 小鼠的骨髓微核试验中，通过口服灌胃以高达 2000mg/kg bw/天的剂量给药，微核多染红细胞（MNPCE）的频率没有显著增加或呈剂量依赖性增加，高剂量抑制了多染红细胞（PCE）与总红细胞的比例。我们的结论是，在预期的使用条件下，岩藻聚糖不存在显著的遗传毒性问题。
