富含岩藻多糖的孢子叶的遗传毒性评估。
Genotoxicity evaluation of fucoidan-rich sporophyll.
作者信息
Jang Miran, Choi Soo-Im, Kim Gun-Hee
机构信息
Department of Food Technology and Nutrition, Inje University, Gimhae, 50834 Republic of Korea.
Department of Institute of Digital Anti-Aging Healthcare, Inje University, Gimhae, 50834 Republic of Korea.
出版信息
Toxicol Res. 2024 Aug 23;41(1):39-46. doi: 10.1007/s43188-024-00258-9. eCollection 2025 Jan.
UNLABELLED
The aim of this study was to investigate genotoxicity of fucoidan-rich sporophyll (FUPS) using a three-component test battery. Our sulfate analysis method showed that FUPS extract contained 14% fucoidan sulfate. The reverse mutation assay showed that the FUPS extract did not increase the number of revertant colonies in any of the five bacterial strains tested, regardless of metabolic activation by S9 mix. Furthermore, FUPS did not induce chromosomal aberrations in the 6-h short-term test with or without S9, as well as in the 24-h continuous test without S9. Finally, bone marrow micronucleus examination of ICR mice at oral doses up to 5000 mg/kg/day did not show a significantly dose-dependent increase in the frequency of micronucleated polychromatic erythrocytes (MNPCEs) or the ratio of polychromatic erythrocytes (PCEs) to total erythrocytes. In conclusion, it was determined that the FUPS extract does not have a significant genotoxic potential under the expected conditions of use.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s43188-024-00258-9.
未标记
本研究的目的是使用三组分测试组合来研究富含岩藻聚糖的孢子叶（FUPS）的遗传毒性。我们的硫酸盐分析方法表明，FUPS提取物含有14%的硫酸岩藻聚糖。回复突变试验表明，无论是否经S9混合物进行代谢活化，FUPS提取物在测试的五种细菌菌株中均未增加回复菌落的数量。此外，在有或无S9的6小时短期试验以及无S9的24小时连续试验中，FUPS均未诱导染色体畸变。最后，对口服剂量高达5000 mg/kg/天的ICR小鼠进行骨髓微核检查，未发现微核多染红细胞（MNPCEs）频率或多染红细胞（PCEs）与总红细胞比例有显著的剂量依赖性增加。总之，确定FUPS提取物在预期使用条件下没有显著的遗传毒性潜力。
补充信息
在线版本包含可在10.1007/s43188-024-00258-9获取的补充材料。
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