Department of Drug Technology, Medical University of Warsaw, Banacha 1 St., 02-097 Warsaw, Poland.
Food Chem Toxicol. 2010 Apr;48(4):1085-91. doi: 10.1016/j.fct.2010.01.030. Epub 2010 Feb 4.
Preparations derived from Lentinula edodes (Berk.) Pegl. mycelium are worldwide used as dietary supplements containing compounds active as immune system enhancers, demonstrating chemopreventive and anticancer activity. L. edodes mycelium enriched with organic forms of selenium like selenized yeast possess putative, higher cancer preventive properties. The objective of this study was to test the effect of enrichment in selenium on antioxidant, reducing and free radical scavenging activity of water and alcohol extracts from mycelium of L. edodes (Berk.). To elucidate the cause of enhanced antioxidant activity of extracts, a preliminary selenium speciation by specific oxido-reduction reaction was performed. Se-enrichment enhanced antioxidant activity, reducing power and free radical scavenging effect of mycelial extracts by almost 100-400%. Increase of activity was particularly high for diluted extracts (concentrations 0.1-0.5 mg/ml). The chemical composition of extracts from both Se-enriched and non-enriched mycelium was compared by determination of polyphenols, proteins, carbohydrates and lipids. Results showed that Se-enrichment enhanced antioxidant activities of mycelial extracts, likely by high amounts of organic Se-compounds (-II oxidation state) and elemental red selenium, and by increased polyphenols content. Our results suggest that Se-enrichment is a good method for enhancement of important activities of human dietary supplements, including Shiitake preparations.
香菇菌丝体的制剂被广泛用作含有免疫系统增强剂活性的膳食补充剂，具有化学预防和抗癌活性。富含有机硒形式（如酵母硒）的香菇菌丝体具有更高的癌症预防特性。本研究的目的是测试硒富集对香菇（Berk.）菌丝体的水和醇提取物的抗氧化、还原和自由基清除活性的影响。为了阐明提取物抗氧化活性增强的原因，通过特定的氧化还原反应进行了初步的硒形态分析。硒富集增强了菌丝体提取物的抗氧化活性、还原能力和自由基清除作用，增强幅度接近 100-400%。对于稀释提取物（浓度为 0.1-0.5mg/ml），活性增加尤其高。通过测定多酚、蛋白质、碳水化合物和脂质，比较了富硒和非富硒菌丝体提取物的化学成分。结果表明，硒富集可能通过大量的有机硒化合物（-II 氧化态）和元素红色硒以及增加的多酚含量，增强了菌丝体提取物的抗氧化活性。我们的结果表明，硒富集是增强包括香菇制剂在内的人类膳食补充剂重要活性的一种很好的方法。
