Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
Department of Clinical Immunology, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, Poland.
Nutrients. 2023 Sep 16;15(18):4015. doi: 10.3390/nu15184015.
Mycelial cultures of , an edible and medicinal mushroom, have been used in our previous research to obtain selenium-containing immunomodulatory preparations. Our current attempts to obtain a new preparation containing both selenium and zinc, two micronutrients necessary for the functioning of the immune system, extended our interest in the simultaneous accumulation of these elements by mycelia growing in media enriched with selenite and zinc(II) ions. Subsequently, we have studied the effects of new mycelium water extracts with different concentrations of selenium and zinc on the activation of T cell fraction in human peripheral blood mononuclear cells (PBMCs). Flow cytometry analysis was used to measure the expression of activation markers on human CD4+ and CD8+ T cells stimulated by anti-CD3 and anti-CD3/CD28 antibodies (Abs). It was demonstrated that statistically significant changes were observed for PD-1 and CD25 antigens on CD8+ T cells. The selenium and zinc content in the examined preparations modified the immunomodulatory activity of mycelial polysaccharides; however, the mechanisms of action of various active ingredients in the mycelial extracts seem to be different.
我们之前的研究中使用了一种可食用和药用蘑菇 的菌丝体培养物来获得含硒的免疫调节剂制剂。为了获得同时含有硒和锌这两种免疫系统功能所必需的微量元素的新制剂，我们目前正在尝试将其添加到富含有亚硒酸盐和锌(II)离子的培养基中，以观察菌丝体的同时积累情况。随后，我们研究了含有不同浓度硒和锌的新 菌丝体水提取物对人外周血单个核细胞（PBMCs）中 T 细胞亚群激活的影响。流式细胞术分析用于测量抗 CD3 和抗 CD3/CD28 抗体（Abs）刺激的人 CD4+和 CD8+T 细胞上激活标志物的表达。结果表明，CD8+T 细胞上的 PD-1 和 CD25 抗原发生了统计学上显著的变化。检查制剂中的硒和锌含量改变了菌丝体多糖的免疫调节活性；然而，菌丝体提取物中各种活性成分的作用机制似乎不同。
