优化脐血造血干细胞的冷冻保存条件。

Optimization of cryopreservation condition for hematopoietic stem cells from umbilical cord blood.

机构信息

Binex Research Institute, Binex Co. Ltd., 480-2, Jangrim-dong, Saha-gu, Busan 604-846, Republic of Korea.

出版信息

Cryobiology. 2010 Jun;60(3):287-92. doi: 10.1016/j.cryobiol.2010.01.007. Epub 2010 Feb 4.

DOI:10.1016/j.cryobiol.2010.01.007

PMID:20138169

Abstract

The conditions for cryopreservation of CD34(+) hematopoietic stem cells (HSC) from umbilical cord blood (UCB) were optimized with a new cryo-medium containing 10% ethylene glycol (EG) and 2% dimethyl sulfoxide (Me(2)SO) using a controlled-rate freezing (CRF) method. After the cryopreservation of mononuclear cells (MNC) from UCB, recoveries of MNC, CD34(+) cells, and total colony-forming units (CFU) were significantly improved compared to those in the control cryo-medium containing 10% Me(2)SO and 2% Dextran-40 (P<0.05). This study shows that the new cryo-medium and CRF method provide better recoveries of MNC, HSC and total CFU than the control cryo-medium and isopropylalcohol freezing (IPA) method. Therefore, this cryo-medium, combined with the CRF method, is valuable for optimizing cryopreservation conditions for HSC from UCB to obtain satisfactory HSC recovery.

摘要

采用含有 10%乙二醇（EG）和 2%二甲基亚砜（Me2SO）的新型冷冻培养基，通过控速冷冻（CRF）方法，优化了从脐血（UCB）中冷冻保存 CD34+造血干细胞（HSC）的条件。与含有 10% Me2SO 和 2%Dextran-40 的对照冷冻培养基相比，UCB 单核细胞（MNC）冷冻保存后，MNC、CD34+细胞和总集落形成单位（CFU）的回收率明显提高（P<0.05）。本研究表明，与对照冷冻培养基和异丙醇冷冻（IPA）方法相比，新型冷冻培养基和 CRF 方法可提供更好的 MNC、HSC 和总 CFU 回收率。因此，这种冷冻培养基与 CRF 方法相结合，对于优化从 UCB 中冷冻保存 HSC 的条件，以获得满意的 HSC 回收率具有重要价值。