Chen Gecai, Yue Aihuan, Ruan Zhongbao, Yin Yigang, Wang Ruzhu, Ren Yin, Zhu Li
Department of Cardiology, Taizhou People's Hospital, 399 Hailing Road, Taizhou, Jiangsu 225300, China.
Stem Cell Research Center, 399 Hailing Road, Taizhou, Jiangsu 225300, China.
Stem Cells Int. 2016;2016:1396783. doi: 10.1155/2016/1396783. Epub 2015 Dec 7.
Purpose. Cryoprotectants (CPA) for stem cells from umbilical cord blood (UCB) have been widely developed based on empirical evidence, but there is no consensus on a standard protocol of preservation of the UCB cells. Methods. In this study, UCB from 115 donors was collected. Each unit of UCB was divided into four equal parts and frozen in different kinds of cryoprotectant as follows: group A, 10% ethylene glycol and 2.0% dimethyl sulfoxide (DMSO) (v/v); group B, 10% DMSO and 2.0% dextran-40; group C, 2.5% DMSO (v/v) + 30 mmol/L trehalose; and group D, without CPA. Results. CD34(+), cell viability, colony forming units (CFUs), and cell apoptosis of pre- and postcryopreservation using three cryoprotectants were analyzed. After thawing, significant differences in CD34(+) count, CFUs, cell apoptosis, and cell viability were observed among the four groups (P < 0.05). Conclusion. The low concentration of DMSO with the addition of trehalose might improve the cryopreservation outcome.
目的。基于经验证据，用于脐带血（UCB）干细胞的冷冻保护剂（CPA）已得到广泛开发，但关于UCB细胞保存的标准方案尚无共识。方法。在本研究中，收集了115名供体的UCB。每个UCB单位被分成四个相等的部分，并在不同种类的冷冻保护剂中冷冻，如下所示：A组，10%乙二醇和2.0%二甲基亚砜（DMSO）（v/v）；B组，10%DMSO和2.0%右旋糖酐-40；C组，2.5%DMSO（v/v）+30 mmol/L海藻糖；D组，无CPA。结果。分析了使用三种冷冻保护剂进行冷冻保存前后的CD34(+)、细胞活力、集落形成单位（CFU）和细胞凋亡情况。解冻后，四组之间在CD34(+)计数、CFU、细胞凋亡和细胞活力方面观察到显著差异（P < 0.05）。结论。添加海藻糖的低浓度DMSO可能会改善冷冻保存效果。
