Département de Chimie, Laboratoire de Chimie Bioorganique, Université Badji Mokhtar, Annaba, Algeria.
J Ethnopharmacol. 2010 May 4;129(1):38-45. doi: 10.1016/j.jep.2010.01.056. Epub 2010 Feb 4.
Renal protection and antiurolithiasic effects of two extracts of Paronychia argentea (PA), a traditional Algerian plant commonly known as Algerian tea, were evaluated. This study was carried out to determine whether the aqueous extract (APA) or the butanolic extract (BPA) of aerial parts could prevent or reduce calculi aggregation in experimental calcium oxalate (Ox) nephrolithiasis in Wistar rats.
The two extracts (APA and BPA) were administrated orally and daily, during 28 days to nephrolithiasic treated rats at the dose of 250, 500 mg/kg b.w. and 10, 20mg/kg b.w. respectively. Body weight, renal index, liver index, serum level of creatinine, uric acid, urea, K(+), Ca(2+), Mg(2+), Na(+) and transaminase (alanine aminotransferase, ALT; aspartate aminotransferase, AST), phosphatase alkaline activity (PAL) were evaluated following the 28 days treatment in rats. In addition histopathological changes in kidney and liver were stained in hematoxylin eosin (HE).
The effect of the extracts could be advantageous in preventing urinary stone retention by reducing renal necrosis and thus inhibit crystal retention. In contradiction with APA, the two doses of BPA attenuated elevation in the serum creatinine (p<0.01) and blood urea levels (p<0.01) (nephroprotective effect). However, the increase in ALT (27%) and PAL (31-51%) serum levels and in the relative liver weights (p<0.01) in the groups treated with doses of APA may indicate that this extract has not a hepatoprotective effect against oxalate toxicity.
The presented data indicate that administration of the butanolic extract of aerial parts to rats with NaOx induced lithiasis, and reduced and prevented the growth of urinary stones in experimental calcium oxalate nephrolithiasis in Wistar rats.
评估两种 Paronychia argentea（PA）提取物（PA）的肾脏保护和抗结石作用。PA 是一种传统的阿尔及利亚植物，通常被称为阿尔及利亚茶，本研究旨在确定其水提物（APA）或正丁醇提物（BPA）是否能预防或减少实验性草酸钙（Ox）肾结石形成大鼠的结石聚集。
将两种提取物（APA 和 BPA）以 250、500mg/kg bw 和 10、20mg/kg bw 的剂量分别口服和每日给药 28 天，用于治疗肾结石的大鼠。在治疗大鼠 28 天后，评估体重、肾脏指数、肝脏指数、血清肌酐、尿酸、尿素、K(+)、Ca(2+)、Mg(2+)、Na(+)和转氨酶（丙氨酸氨基转移酶，ALT；天冬氨酸氨基转移酶，AST）、碱性磷酸酶（PAL）活性。此外，用苏木精-伊红（HE）染色观察肾脏和肝脏的组织病理学变化。
提取物的作用可能有利于通过减少肾坏死来预防尿结石的保留，并因此抑制晶体保留。与 APA 相反，BPA 的两个剂量降低了血清肌酐（p<0.01）和血液尿素水平（p<0.01）的升高（肾保护作用）。然而，用 APA 剂量治疗的组的血清 ALT（27%）和 PAL（31-51%）水平升高以及相对肝重增加（p<0.01）可能表明该提取物对草酸毒性没有肝保护作用。
提供的数据表明，给予患有 NaOx 诱导结石形成的大鼠正丁醇提取物，并减少和预防了实验性草酸钙肾结石形成大鼠的尿结石生长。
