Singh Mohan, Sandhir Rajat, Kiran Ravi
Department of Biochemistry, Basic Medical Science Block, Panjab University, Chandigarh 160014, India.
Exp Toxicol Pathol. 2011 Mar;63(3):269-76. doi: 10.1016/j.etp.2010.01.005. Epub 2010 Feb 6.
In the present investigation, the effect of atrazine on antioxidant enzymes and body weight was studied in male Wistar rats. Atrazine (300 mg/kgbw) was administered by gavage for 7, 14 and 21 days. A significant increase in hepatic lipid peroxidation (LPO) was observed following atrazine administration. Vitamin E treatment (100 mg/kgbw), on the otherhand, attenuated atrazine-induced LPO in liver. In addition, vitamin E treatment restored the GSH content and glucose-6-phosophate dehydrogenase activity that was found to be lowered after atrazine administration. The activities of antioxidant enzymes: superoxide dismutase, catalase, glutathione peroxidase and glutathione-s-transferase were significantly increased following atrazine administration and vitamin E treatment could restore these activities. In conclusion, the results of the study demonstrate that atrazine induces oxidative stress in terms of enhanced lipid peroxidation. However, vitamin E treatment ameliorated the effects of atrazine suggesting it as potential antioxidant against atrazine-induced oxidative stress.
在本研究中，研究了阿特拉津对雄性Wistar大鼠抗氧化酶和体重的影响。通过灌胃给予阿特拉津（300 mg/kg体重），持续7、14和21天。给予阿特拉津后，观察到肝脏脂质过氧化（LPO）显著增加。另一方面，维生素E处理（100 mg/kg体重）减轻了阿特拉津诱导的肝脏LPO。此外，维生素E处理恢复了谷胱甘肽（GSH）含量和葡萄糖-6-磷酸脱氢酶活性，这些在给予阿特拉津后被发现降低。抗氧化酶超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽-S-转移酶的活性在给予阿特拉津后显著增加，维生素E处理可以恢复这些活性。总之，研究结果表明，阿特拉津在脂质过氧化增强方面诱导氧化应激。然而，维生素E处理改善了阿特拉津的影响，表明它是对抗阿特拉津诱导的氧化应激的潜在抗氧化剂。
