Biochemical Genetics Laboratory, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
Mol Genet Metab. 2010 May;100(1):103-4. doi: 10.1016/j.ymgme.2010.01.006. Epub 2010 Jan 21.
We report the artifactual elevation of homogentisic acid (HGA) in urine from alkaptonuric patients after replacing the creatinine method (Jaffe reaction) in our laboratory with an automated enzymatic method. Samples with elevated HGA by GC-MS had lower creatinine values as determined by the enzymatic method than by the Jaffe reaction. The low creatinine values were due to interference by HGA in the enzymatic method. The enzymatic method is unsuitable for creatinine determination in urine of patients with alkaptonuria.
我们报告了在实验室将肌酐法（Jaffe 反应）替换为自动酶法后，尿中天冬氨酸（HGA）在黑尿酸症患者中的人为升高。用 GC-MS 测定 HGA 升高的样品，用酶法测定的肌酐值比 Jaffe 反应低。低肌酐值是由于 HGA 对酶法的干扰。该酶法不适合用于黑尿酸症患者尿液中肌酐的测定。
