采用高效亲和色谱法分析利多卡因与血清蛋白的相互作用。
Analysis of lidocaine interactions with serum proteins using high-performance affinity chromatography.
机构信息
Chemistry Department, University of Nebraska, 704 Hamilton Hall, Lincoln, NE 68588-0304, USA.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Mar 1;878(7-8):705-8. doi: 10.1016/j.jchromb.2010.01.016. Epub 2010 Jan 25.
Abstract
High-performance affinity chromatography was used to study binding by the drug lidocaine to human serum albumin (HSA) and alpha(1)-acid glycoprotein (AGP). AGP had strong binding to lidocaine, with an association equilibrium constant (K(a)) of 1.1-1.7 x 10(5) M(-1) at 37 degrees C and pH 7.4. Lidocaine had weak to moderate binding to HSA, with a K(a) in the range of 10(3) to 10(4) M(-1). Competitive experiments with site selective probes showed that lidocaine was interacting with Sudlow site II of HSA and the propranolol site of AGP. These results agree with previous observations in the literature and provide a better quantitative understanding of how lidocaine binds to these serum proteins and is transported in the circulation. This study also demonstrates how HPAC can be used to examine the binding of a drug with multiple serum proteins and provide detailed information on the interaction sites and equilibrium constants that are involved in such processes.
摘要
高效亲和色谱法用于研究药物利多卡因与人血清白蛋白（HSA）和α(1)-酸性糖蛋白（AGP）的结合。AGP 与利多卡因具有很强的结合能力，在 37°C 和 pH 7.4 时的缔合平衡常数（K(a)）为 1.1-1.7×10(5)M(-1)。利多卡因与 HSA 的结合能力较弱到中等，K(a)在 10(3)到 10(4)M(-1)范围内。与位点选择性探针的竞争实验表明，利多卡因与 HSA 的 Sudlow 位点 II 和 AGP 的普萘洛尔位点相互作用。这些结果与文献中的先前观察结果一致，并提供了对利多卡因与这些血清蛋白结合以及在循环中运输的更好的定量理解。本研究还展示了 HPAC 如何用于检查具有多种血清蛋白的药物的结合，并提供有关涉及此类过程的相互作用位点和平衡常数的详细信息。
相似文献
1
Analysis of lidocaine interactions with serum proteins using high-performance affinity chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Mar 1;878(7-8):705-8. doi: 10.1016/j.jchromb.2010.01.016. Epub 2010 Jan 25.
2
High-performance affinity chromatography and the analysis of drug interactions with modified proteins: binding of gliclazide with glycated human serum albumin.
Anal Bioanal Chem. 2011 Nov;401(9):2811-9. doi: 10.1007/s00216-011-5382-8. Epub 2011 Sep 16.
3
Analysis of multi-site drug-protein interactions by high-performance affinity chromatography: Binding by glimepiride to normal or glycated human serum albumin.
J Chromatogr A. 2015 Aug 21;1408:133-44. doi: 10.1016/j.chroma.2015.07.012. Epub 2015 Jul 6.
4
Buffer and pH effects on propranolol binding by human albumin and alpha 1-acid glycoprotein.
J Pharm Pharmacol. 1988 Jul;40(7):459-63. doi: 10.1111/j.2042-7158.1988.tb05277.x.
5
Binding of tolbutamide to glycated human serum albumin.
J Pharm Biomed Anal. 2011 Jan 25;54(2):426-32. doi: 10.1016/j.jpba.2010.09.003. Epub 2010 Sep 15.
6
Studies of verapamil binding to human serum albumin by high-performance affinity chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Dec 1;876(1):69-75. doi: 10.1016/j.jchromb.2008.10.022. Epub 2008 Oct 21.
7
Chromatographic studies of drug interactions with alpha-acid glycoprotein by ultrafast affinity extraction and peak profiling.
J Chromatogr A. 2017 May 12;1497:92-101. doi: 10.1016/j.chroma.2017.03.056. Epub 2017 Mar 23.
8
Analysis of glipizide binding to normal and glycated human serum albumin by high-performance affinity chromatography.
Anal Bioanal Chem. 2015 Jul;407(18):5309-21. doi: 10.1007/s00216-015-8688-0. Epub 2015 Apr 26.
9
Analysis of drug interactions with modified proteins by high-performance affinity chromatography: binding of glibenclamide to normal and glycated human serum albumin.
J Chromatogr A. 2012 Nov 23;1265:114-22. doi: 10.1016/j.chroma.2012.09.091. Epub 2012 Oct 8.
10
Characterization of the binding of sulfonylurea drugs to HSA by high-performance affinity chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jun 1;878(19):1590-8. doi: 10.1016/j.jchromb.2010.04.019.
引用本文的文献
1
Changes in plasma alpha-1 acid glycoprotein following hemorrhagic trauma: Possible role in dose differences of ALM drug therapy in rat and pig resuscitation.
Pharmacol Res Perspect. 2023 Oct;11(5):e01133. doi: 10.1002/prp2.1133.
2
Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?
Int J Mol Sci. 2023 May 9;24(10):8472. doi: 10.3390/ijms24108472.
3
Kinetic Analysis of Lidocaine Elimination by Pig Liver Cells Cultured in 3D Multi-Compartment Hollow Fiber Membrane Network Perfusion Bioreactors.
Bioengineering (Basel). 2021 Jul 23;8(8):104. doi: 10.3390/bioengineering8080104.
4
Characterization of solution-phase drug-protein interactions by ultrafast affinity extraction.
Methods. 2018 Aug 15;146:46-57. doi: 10.1016/j.ymeth.2018.02.021. Epub 2018 Mar 3.
5
High performance affinity chromatography and related separation methods for the analysis of biological and pharmaceutical agents.
Analyst. 2018 Jan 15;143(2):374-391. doi: 10.1039/c7an01469d.
6
Chromatographic studies of drug interactions with alpha-acid glycoprotein by ultrafast affinity extraction and peak profiling.
J Chromatogr A. 2017 May 12;1497:92-101. doi: 10.1016/j.chroma.2017.03.056. Epub 2017 Mar 23.
7
Analysis of free drug fractions in serum by ultrafast affinity extraction and two-dimensional affinity chromatography using α1-acid glycoprotein microcolumns.
J Chromatogr A. 2016 Feb 5;1432:49-57. doi: 10.1016/j.chroma.2015.12.084. Epub 2016 Jan 4.
8
Entrapment of alpha1-acid glycoprotein in high-performance affinity columns for drug-protein binding studies.
J Chromatogr B Analyt Technol Biomed Life Sci. 2016 May 15;1021:188-196. doi: 10.1016/j.jchromb.2015.11.021. Epub 2015 Nov 27.
9
Pharmaceutical and biomedical applications of affinity chromatography: recent trends and developments.
J Pharm Biomed Anal. 2012 Oct;69:93-105. doi: 10.1016/j.jpba.2012.01.004. Epub 2012 Jan 14.
10
Calculation of normalized drug concentrations in the presence of altered plasma protein binding.
Clin Pharmacokinet. 2012 Jan 1;51(1):55-68. doi: 10.2165/11595650-000000000-00000.
本文引用的文献
1
The importance of plasma protein binding in drug discovery.
Expert Opin Drug Discov. 2007 Jan;2(1):51-64. doi: 10.1517/17460441.2.1.51.
2
Development of an affinity silica monolith containing alpha1-acid glycoprotein for chiral separations.
J Chromatogr A. 2007 May 18;1149(2):294-304. doi: 10.1016/j.chroma.2007.03.063. Epub 2007 Mar 25.
3
An integrated in silico analysis of drug-binding to human serum albumin.
J Chem Inf Model. 2006 Nov-Dec;46(6):2709-24. doi: 10.1021/ci600274f.
4
Evaluation of a hydrazide-linked alpha1-acid glycoprotein chiral stationary phase: separation of R- and S-propranolol.
J Sep Sci. 2006 Jul;29(10):1412-22. doi: 10.1002/jssc.200600051.
5
Drug binding to human serum albumin: abridged review of results obtained with high-performance liquid chromatography and circular dichroism.
Chirality. 2006 Sep;18(9):667-79. doi: 10.1002/chir.20301.
6
Chromatographic analysis of carbamazepine binding to human serum albumin. II. Comparison of the Schiff base and N-hydroxysuccinimide immobilization methods.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Jun 6;837(1-2):138-46. doi: 10.1016/j.jchromb.2006.03.062. Epub 2006 May 9.
7
In silico ADME modelling 2: computational models to predict human serum albumin binding affinity using ant colony systems.
Bioorg Med Chem. 2006 Jun 15;14(12):4118-29. doi: 10.1016/j.bmc.2006.02.008. Epub 2006 Feb 28.
8
A molecular functional study on the interactions of drugs with plasma proteins.
Drug Metab Pharmacokinet. 2005 Oct;20(5):309-23. doi: 10.2133/dmpk.20.309.
9
Immobilization of alpha(1)-acid glycoprotein for chromatographic studies of drug-protein binding.
Anal Biochem. 2005 Nov 15;346(2):300-10. doi: 10.1016/j.ab.2005.08.025. Epub 2005 Sep 19.
10
Chromatographic determination of free lidocaine and its active metabolites in plasma from patients under epidural anesthesia.
Int J Clin Pharmacol Ther. 2002 Nov;40(11):493-8. doi: 10.5414/cpp40493.
Zotero 插件