Department of Cellular Biology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil.
Exp Parasitol. 2010 Jul;125(3):271-8. doi: 10.1016/j.exppara.2010.02.002. Epub 2010 Feb 6.
Experimental cerebral malaria is a neuroinflammatory condition that results from the host immune response to the parasite. Using intravital microscopy, we investigated leukocyte recruitment in the brain microcirculation and the temporal relationship of this process to the behavioral changes observed in Plasmodium berghei (strain ANKA)-infected C57Bl/6 mice. We found that leukocyte recruitment was increased from day 5 post-infection (p.i.) onwards. Histopathological changes and increased levels of inflammatory cytokines in the brain were also observed. Behavioral performance evaluated by the SHIRPA protocol showed functional impairment from day 6 p.i. onwards. Thus, early leukocyte migration into the brain and associated inflammatory changes may be involved in neurological impairment in parasite-infected C57Bl/6 mice.
实验性脑疟疾是一种由宿主对寄生虫的免疫反应引起的神经炎症状态。使用活体显微镜，我们研究了脑微循环中的白细胞募集情况，以及该过程与感染疟原虫（安卡拉株）的 C57Bl/6 小鼠中观察到的行为变化之间的时间关系。我们发现白细胞募集从感染后第 5 天（p.i.）开始增加。大脑中的组织病理学变化和炎症细胞因子水平升高也观察到。通过 SHIRPA 方案评估的行为表现显示从感染后第 6 天开始出现功能障碍。因此，早期白细胞向大脑迁移和相关的炎症变化可能与感染寄生虫的 C57Bl/6 小鼠的神经损伤有关。
