Department of Aquaculture, College of Life Sciences, National Taiwan Ocean University, Keelung 202, Taiwan, ROC.
Fish Shellfish Immunol. 2010 May-Jun;28(5-6):764-73. doi: 10.1016/j.fsi.2010.01.023. Epub 2010 Feb 6.
White shrimp Litopenaeus vannamei which had been injected with the hot-water extract of Spirulina platensis at 6, 10, and 20 microg g(-1), or immersed in aerated seawater containing extract at 200, 400, and 600 mg L(-1) were challenged with Vibrio alginolyticus at 1.5 x 10(6) or 1.4 x 10(6) colony-forming units (cfu) shrimp(-1), and then placed in seawater. Survival rates of shrimp that received the extract of S. platensis at 6-20 microg g(-1), and those of shrimp immersed in seawater containing the extract at 400 and 600 mg L(-1) were significantly higher than those of control shrimp after 24-96 and 48-96 h, respectively. In a separate experiment, the hyaline cell (HC) count, granular cell (GC, including semi-granular cell) count, total haemocyte count (THC), phenoloxidase (PO) activity, respiratory burst (RB), superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity, and lysozyme activity were measured when shrimp were injected with the extract at 6, 10, and 20 microg g(-1), and immersed in seawater containing the extract at 200, 400, and 600 mg L(-1). These parameters directly increased with the concentration, and significantly increased when shrimp were immersed in the seawater containing the extract at 0.5-4 h. L. vannamei that received all doses of the extract via injection or via immersion all had increased phagocytic activity and clearance efficiency to V. alginolyticus at 12-72 h and 3-4 h, respectively. It was concluded that L. vannamei that received the hot-water extract of S. platensis had enhanced innate immunity and increased resistance against V. alginolyticus infection.
凡纳滨对虾（Litopenaeus vannamei）经螺旋藻（Spirulina platensis）热水提取物以 6、10 和 20 μg g(-1)的剂量注射，或浸泡在含有 200、400 和 600 mg L(-1)提取物的充气海水中，然后用 1.5×10(6)或 1.4×10(6)cfu 虾(-1)的溶藻弧菌（Vibrio alginolyticus）进行攻毒，然后放入海水中。在 24-96 和 48-96 h 后，接受螺旋藻提取物剂量为 6-20 μg g(-1)的虾和浸泡在含有提取物的海水中 400 和 600 mg L(-1)的虾的存活率明显高于对照组虾。在另一个实验中，当虾被注射提取物 6、10 和 20 μg g(-1)，或浸泡在含有提取物 200、400 和 600 mg L(-1)的海水中时，测量透明细胞（HC）计数、颗粒细胞（GC，包括半颗粒细胞）计数、总血细胞计数（THC）、酚氧化酶（PO）活性、呼吸爆发（RB）、超氧化物歧化酶（SOD）活性、谷胱甘肽过氧化物酶（GPx）活性和溶菌酶活性。这些参数与浓度直接相关，当虾在含有提取物的海水中浸泡 0.5-4 h 时，显著增加。通过注射或浸泡接受提取物所有剂量的凡纳滨对虾在 12-72 h 和 3-4 h 时对溶藻弧菌的吞噬活性和清除效率均增加。结果表明，凡纳滨对虾接受螺旋藻热水提取物后，先天免疫增强，对溶藻弧菌感染的抵抗力增强。
