National Institute of Oceanography and Fisheries (NIOF), Cairo, Egypt.
Fish Production Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.
PLoS One. 2024 Jun 18;19(6):e0300748. doi: 10.1371/journal.pone.0300748. eCollection 2024.
The current study aimed to assess the influence of dietary inclusion of cyanobacterium Arthrospira platensis NIOF17/003 as a dry material and as a free-lipid biomass (FL) on the growth performance, body composition, redox status, immune responses, and gene expression of whiteleg shrimp, Litopenaeus vannamei postlarvae. L. vannamei were fed five different supplemented diets; the first group was fed on an un-supplemented diet as a negative control group (C-N), the second group was fed on a commercial diet supplemented with 2% of A. platensis complete biomass as a positive control group (C-P20), whereas, the three remaining groups were fed on a commercial diet supplemented with graded amounts of FL at 1%, 2%, and 3% (FL10, FL20, and FL30, respectively). The obtained results indicated that the diet containing 1% FL significantly increased the growth performance, efficiency of consumed feed, and survival percentage of L. vannamei compared to both C-N and C-P20 groups. As for the carcass analysis, diets containing A. platensis or its FL at higher levels significantly increased the protein, lipid, and ash content compared to the C-N group. Moreover, the shrimp group fed on C-P20 and FL10 gave significantly stimulated higher digestive enzyme activities compared with C-N. The shrimp fed C-P20 or FL exhibited higher innate immune responses and promoted their redox status profile. Also, the shrimp fed a low FL levels significantly upregulated the expression of both the peroxiredoxin (Prx) and prophenoloxidase (PPO1) genes than those receiving C-N. The current results recommended that dietary supplementation with 1% FL is the most effective treatment in promoting the performance and immunity of whiteleg shrimp.
本研究旨在评估以干粉形式和游离脂生物质（FL）形式添加食用蓝藻节旋藻 Arthrospira platensis NIOF17/003 对凡纳滨对虾（Litopenaeus vannamei）幼虾生长性能、体组成、氧化还原状态、免疫反应和基因表达的影响。L. vannamei 被喂食五种不同的补充饲料；第一组喂食未补充的饲料作为阴性对照组（C-N），第二组喂食补充有 2%完整节旋藻生物量的商业饲料作为阳性对照组（C-P20），而其余三组则喂食补充有 1%、2%和 3%FL 的商业饲料（FL10、FL20 和 FL30）。结果表明，与 C-N 和 C-P20 组相比，含 1%FL 的饲料显著提高了 L. vannamei 的生长性能、饲料效率和成活率。就胴体分析而言，与 C-N 组相比，含节旋藻或其 FL 较高水平的饲料显著增加了蛋白质、脂肪和灰分含量。此外，与 C-N 组相比，喂食 C-P20 和 FL10 的虾的消化酶活性显著提高。喂食 C-P20 或 FL 的虾表现出更高的先天免疫反应，并改善了其氧化还原状态。此外，低水平 FL 喂养的虾显著上调了过氧化物酶（Prx）和原酚氧化酶（PPO1）基因的表达，而这些基因在接受 C-N 喂养的虾中表达水平较低。目前的结果表明，在促进凡纳滨对虾的性能和免疫力方面，1%FL 的饮食补充是最有效的治疗方法。
