Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, 205 North Mathews Avenue, Urbana, Illinois 61801, USA.
Appl Environ Microbiol. 2010 Apr;76(7):2218-24. doi: 10.1128/AEM.02183-09. Epub 2010 Feb 5.
Current agricultural practices involve inclusion of antimicrobials in animal feed and result in manure containing antimicrobials and antimicrobial-resistant microorganisms. This work evaluated the effects of land application of swine manure on the levels of tetracycline, macrolide, and lincosamide antimicrobials and on macrolide, lincosamide, and streptogramin B (MLS(B)) resistance in field soil samples and laboratory soil batch tests. MLS(B) and tetracycline antimicrobials were quantified after solid-phase extraction using liquid chromatography-tandem mass spectrometry. The prevalence of the ribosomal modification responsible for MLS(B) resistance in the same samples was quantified using fluorescence in situ hybridization. Macrolide antimicrobials were not detected in soil samples, while tetracyclines were detected, suggesting that the latter compounds persist in soil. No significant differences in ribosomal methylation or presumed MLS(B) resistance were observed when amended and unamended field soils were compared, although a transient (<20-day) increase was observed in most batch tests. Clostridium cluster XIVa accounted for the largest fraction of resistant bacteria identified in amended soils. Overall, this study did not detect a persistent increase in the prevalence of MLS(B) resistance due to land application of treated swine manure.
当前的农业实践包括在动物饲料中添加抗生素，导致粪便中含有抗生素和抗微生物的微生物。这项工作评估了猪粪土地应用对田间土壤样品和实验室土壤批量试验中四环素、大环内酯和林可酰胺类抗生素水平以及大环内酯类、林可酰胺类和链阳性菌素 B（MLS(B)) 抗性的影响。使用液相色谱-串联质谱法通过固相萃取定量 MLS(B) 和四环素类抗生素。使用荧光原位杂交定量相同样品中负责 MLS(B) 抗性的核糖体修饰的流行率。土壤样品中未检测到大环内酯类抗生素，而检测到四环素类抗生素，表明后者化合物在土壤中持续存在。虽然在大多数批量试验中观察到短暂（<20 天）增加，但比较添加和未添加的田间土壤时，核糖体甲基化或假定的 MLS(B) 抗性没有显着差异。梭菌簇 XIVa 占添加土壤中鉴定出的抗性细菌的最大部分。总体而言，这项研究没有发现由于处理过的猪粪土地应用而导致 MLS(B) 抗性流行率持续增加。
