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嗜热羧基放线菌内切果胶酸裂解酶编码基因的克隆

Cloning of the gene encoding an endo-acting pectate lyase from Streptomyces thermocarboxydus.

作者信息

Tonouchi Akio, Hara Yuichi, Umehara Ryousuke, Sanuki Tamotsu, Fukusawa Takuma, Miyairi Kazuo

机构信息

Faculty of Agriculture and Life Science, Hirosaki University, Aomori, Japan.

出版信息

Biosci Biotechnol Biochem. 2010;74(2):433-6. doi: 10.1271/bbb.90693. Epub 2010 Feb 7.

DOI:10.1271/bbb.90693
PMID:20139595
Abstract

An endo-acting family 3 pectate lyase (PL I) gene from Streptomyces thermocarboxydus was cloned and expressed in Escherichia coli. The deduced PL I sequence had highest similarity to a putative pectate lyase of S. coelicolor. Recombinant PL I had significantly lower specific activity, probably a result of incorrect folding, as indicated by circular dichroism spectra analysis.

摘要

克隆了来自嗜热羧基放线菌的一种内切作用的家族3果胶酸裂解酶（PL I）基因，并在大肠杆菌中进行表达。推导的PL I序列与天蓝色链霉菌的一种假定果胶酸裂解酶具有最高的相似性。如圆二色光谱分析所示，重组PL I的比活性显著较低，这可能是折叠错误的结果。

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