Department of Molecular Evolution, Evolutionary Biology Center, Uppsala University, Uppsala, Sweden.
Cell Cycle. 2010 Feb 15;9(4):794-806. Epub 2010 Feb 17.
The temporal and spatial organization of the chromosome replication, genome segregation and cell division processes is less well understood in species belonging to the Archaea, than in those from the Bacteria and Eukarya domains. Novel insights into the regulation and key components of the Sulfolobus acidocaldarius cell cycle have been obtained through genome-wide analysis of cell cycle-specific gene expression, followed by cloning and characterization of gene products expressed at different cell cycle stages. Here, the results of the transcript profiling are further explored, and potential key players in archaeal cell cycle progression are highlighted in an evolutionary context, by comparing gene expression patterns and gene conservation between three selected microbial species from different domains of life. We draw attention to novel putative nucleases and helicases implicated in DNA replication, recombination and repair, as well as to potential genome segregation factors. Focus is also placed upon regulatory features, including transcription factors and protein kinases inferred to be involved in the execution of specific cell cycle stages, and regulation through metabolic coupling is discussed.
在古菌中，染色体复制、基因组分离和细胞分裂过程的时空组织的理解程度不如细菌和真核生物领域的理解程度。通过对细胞周期特异性基因表达进行全基因组分析，随后克隆和鉴定不同细胞周期阶段表达的基因产物，获得了对 Sulfolobus acidocaldarius 细胞周期调控和关键成分的新认识。在这里，通过比较来自生命不同领域的三个选定微生物物种之间的基因表达模式和基因保守性，进一步探讨了转录谱分析的结果，并在进化背景下突出了参与古菌细胞周期进程的潜在关键因素。我们注意到新的推定核酸内切酶和解旋酶，它们涉及 DNA 复制、重组和修复，以及潜在的基因组分离因子。还重点关注了包括转录因子和蛋白激酶在内的调节特征，这些因子被认为参与特定细胞周期阶段的执行，并且讨论了通过代谢偶联进行的调节。
