Department of Biological Sciences, Seoul National University, Seoul, Korea.
Cell Cycle. 2010 Feb 15;9(4):774-83. Epub 2010 Feb 9.
CEP 215 is a human orthologue of Drosophila centrosomin which is a core centrosome component for the pericentriolar matrix protein recruitment. Recent investigations revealed that CEP 215 is required for centrosome cohesion, centrosomal attachment of the gamma-TuRC, and microtubule dynamics. However, it remains obscure how CEP 215 functions for recruitment of the centrosomal proteins during the centrosome cycle. Here, we investigated a role of CEP 215 during mitosis. Knockdown of CEP 215 resulted in characteristic mitotic phenotypes, including monopolar spindle formation, a decrease in distance between the spindle pole pair, and detachment of the centrosomes from the spindle poles. We noticed that CEP 215 is critical for centrosomal localization of dynein throughout the cell cycle. As a consequence, the selective centrosomal proteins were not recruited to the centrosome properly. Finally, the centrosomal localization of CEP 215 also depends on the dynein-dynactin complex. Based on the results, we propose that CEP 215 regulates a dynein-dependent transport of the pericentriolar matrix proteins during the centrosome maturation.
CEP215 是果蝇中心体蛋白的人类同源物，是募集中心体周围基质蛋白所必需的核心中心体成分。最近的研究表明，CEP215 对于中心体黏合、γ-TuRC 的中心体附着以及微管动力学都是必需的。然而，CEP215 在中心体蛋白募集过程中如何在中心体周期中发挥作用仍然不清楚。在这里，我们研究了 CEP215 在有丝分裂过程中的作用。CEP215 的敲低导致了特征性的有丝分裂表型，包括单极纺锤体的形成、纺锤体极对之间距离的减少以及中心体与纺锤体极的分离。我们注意到，CEP215 对于整个细胞周期中中心体定位的动力蛋白至关重要。因此，选择的中心体蛋白不能正确地被募集到中心体。最后，CEP215 的中心体定位也依赖于动力蛋白-动力素复合物。基于这些结果，我们提出 CEP215 调节中心体成熟过程中中心体周围基质蛋白的依赖于动力蛋白的运输。
