Departments of Medicine and Radiology, Stanford University School of Medicine, Stanford, California, USA.
Nat Methods. 2010 Mar;7(3):197-9. doi: 10.1038/nmeth.1426. Epub 2010 Feb 7.
Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.
由于插入突变的风险，病毒转导已越来越多地被非病毒方法所取代，以生成诱导多能干细胞 (iPSC)。我们报告了使用“微环”DNA 的情况，这是一种不含细菌 DNA 且能够在细胞中高效表达的载体类型，可用于达到这一目的。在此，我们使用单个微环载体从成人脂肪干细胞中生成无转基因 iPSC。
