Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, Moscow, Russia.
Genome. 2010 Feb;53(2):125-37. doi: 10.1139/g09-089.
The chromosome set of Avena macrostachya Balansa ex Coss. et Durieu was analyzed using C-banding and fluorescence in situ hybridization with 5S and 18S-5.8S-26S rRNA gene probes, and the results were compared with the C-genome diploid Avena L. species. The location of major nucleolar organizer regions and 5S rDNA sites on different chromosomes confirmed the affiliation of A. macrostachya with the C-genome group. However, the symmetric karyotype, the absence of "diffuse heterochromatin" and the location of large C-band complexes in proximal chromosome regions pointed to an isolated position of A. macrostachya from other Avena species. Based on the distribution of rDNA loci on the C-genome chromosomes of diploid and polyploid Avena species, we propose a model of the chromosome alterations that occurred during the evolution of oat species.
利用 C-带和 5S 和 18S-5.8S-26S rRNA 基因探针的荧光原位杂交技术，对大穗野燕麦染色体组进行了分析，并与 C 基因组二倍体燕麦属物种进行了比较。不同染色体上主要核仁组织者区域和 5S rDNA 位点的位置证实了大穗野燕麦与 C 基因组组的隶属关系。然而，对称的核型、缺乏“弥散异染色质”以及近端染色体区域大 C 带复合物的位置，表明大穗野燕麦与其他燕麦物种的孤立地位。基于二倍体和多倍体燕麦物种 C 基因组染色体上 rDNA 位点的分布，我们提出了燕麦属物种进化过程中发生的染色体改变模型。
