Chen Xiaowu, Shi Zhiyi
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
Bioinform Biol Insights. 2009 Jun 4;3:29-35. doi: 10.4137/bbi.s2287.
Using zebrafish intestinal fatty acid-binding protein 2 (FABP2) mRNA sequence as the initial query probe, four highly homologous Paralichthys olivaceus EST sequences were retrieved from Genbank database. The assembled full-length cDNA contains the open reading frame of P. olivaceus FABP2 gene, which was validated by subsequent RT-PCR cloning. In the coding region, the average GC content is 56%, but it would reach 76.8% if only counting for the third base of the codons. The deduced P. olivaceus FABP2 polypeptide contains 132 amino acids (aa), with a predicted molecular size of 15.3 kD and pI at 6.74. This protein multiple-alignment has shown that this peptide is 75.7% identical to the corresponding homologous protein in Danio rerio. Among the 7 aa that are essential for FABP2 function, 3 were found to be conserved among P. olivaceus, Danio rerio, Tetraodon nigroviridi, Rattus norvegicus, and Homo sapiens. The study provides essential information on molecular evolution and function of FABP family.
以斑马鱼肠道脂肪酸结合蛋白2（FABP2）mRNA序列作为初始查询探针，从Genbank数据库中检索到4条高度同源的牙鲆EST序列。组装后的全长cDNA包含牙鲆FABP2基因的开放阅读框，随后通过RT-PCR克隆进行了验证。在编码区，平均GC含量为56%，但仅计算密码子的第三个碱基时，GC含量可达76.8%。推导的牙鲆FABP2多肽含有132个氨基酸（aa），预测分子量为15.3 kD，pI为6.74。该蛋白多序列比对显示，该肽与斑马鱼中相应的同源蛋白具有75.7%的同一性。在FABP2功能所必需的7个氨基酸中，发现牙鲆、斑马鱼、黑点青鳉、褐家鼠和智人中的3个氨基酸是保守的。该研究提供了FABP家族分子进化和功能的重要信息。
