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不同亚型禽流感病毒重组血凝素蛋白的抗原特性分析。

Antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes.

机构信息

Institute of Virology and Immunoprophylaxis (IVI), Mittelhaeusern, Switzerland.

出版信息

PLoS One. 2010 Feb 5;5(2):e9097. doi: 10.1371/journal.pone.0009097.

DOI:10.1371/journal.pone.0009097
PMID:20140098
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2816723/
Abstract

Since the advent of highly pathogenic variants of avian influenza virus (HPAIV), the main focus of avian influenza research has been the characterization and detection of HPAIV hemagglutinin (HA) from H5 and H7 subtypes. However, due to the high mutation and reassortation rate of influenza viruses, in theory any influenza strain may acquire increased pathogenicity irrespective of its subtype. A comprehensive antigenic characterization of influenza viruses encompassing all 16 HA and 9 neuraminidase subtypes will provide information useful for the design of differential diagnostic tools, and possibly, vaccines. We have expressed recombinant HA proteins from 3 different influenza virus HA subtypes in the baculovirus system. These proteins were used to generate polyclonal rabbit antisera, which were subsequently employed in epitope scanning analysis using peptide libraries spanning the entire HA. Here, we report the identification and characterization of linear, HA subtype-specific as well as inter subtype-conserved epitopes along the HA proteins. Selected subtype-specific epitopes were shown to be suitable for the differentiation of anti-HA antibodies in an ELISA.

摘要

自高致病性禽流感病毒 (HPAIV) 出现以来，禽流感研究的主要重点一直是对 H5 和 H7 亚型的 HPAIV 血凝素 (HA) 进行特征描述和检测。然而，由于流感病毒的高突变和重配率，理论上任何流感株都可能获得更高的致病性，而与其亚型无关。对涵盖所有 16 种 HA 和 9 种神经氨酸酶亚型的流感病毒进行全面的抗原特征描述，将为设计差异化诊断工具提供有用的信息，并且可能还为疫苗设计提供有用的信息。我们已经在杆状病毒系统中表达了来自 3 种不同流感病毒 HA 亚型的重组 HA 蛋白。这些蛋白被用于产生多克隆兔抗血清，随后使用跨越整个 HA 的肽文库进行表位扫描分析。在这里，我们报告了在 HA 蛋白上鉴定和描述线性、HA 亚型特异性以及亚型间保守表位的情况。已证明选定的亚型特异性表位适合在 ELISA 中区分抗 HA 抗体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2974/2816723/cb8474de7f16/pone.0009097.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2974/2816723/68aafeadd51b/pone.0009097.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2974/2816723/740688445cbf/pone.0009097.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2974/2816723/97db995056f7/pone.0009097.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2974/2816723/b1c8dca06b51/pone.0009097.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2974/2816723/6419687f32c2/pone.0009097.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2974/2816723/d0f5dd5c9f5b/pone.0009097.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2974/2816723/cb8474de7f16/pone.0009097.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2974/2816723/68aafeadd51b/pone.0009097.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2974/2816723/740688445cbf/pone.0009097.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2974/2816723/97db995056f7/pone.0009097.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2974/2816723/b1c8dca06b51/pone.0009097.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2974/2816723/6419687f32c2/pone.0009097.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2974/2816723/d0f5dd5c9f5b/pone.0009097.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2974/2816723/cb8474de7f16/pone.0009097.g007.jpg

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本文引用的文献

1
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Vet Microbiol. 2010 Jan 27;140(3-4):237-45. doi: 10.1016/j.vetmic.2009.08.022. Epub 2009 Aug 26.
2
Antigenic fingerprinting of H5N1 avian influenza using convalescent sera and monoclonal antibodies reveals potential vaccine and diagnostic targets.利用恢复期血清和单克隆抗体对H5N1禽流感进行抗原指纹分析，揭示了潜在的疫苗和诊断靶点。
PLoS Med. 2009 Apr 21;6(4):e1000049. doi: 10.1371/journal.pmed.1000049.
3
A gene-based avian influenza vaccine in poultry.
Construction of recombinant baculoviruses expressing hemagglutinin of H5N1 avian influenza and research on the immunogenicity.
表达H5N1禽流感血凝素的重组杆状病毒的构建及免疫原性研究
Sci Rep. 2016 Apr 11;6:24290. doi: 10.1038/srep24290.
4
Getting more out of less--a quantitative serological screening tool for simultaneous detection of multiple influenza A hemagglutinin-types in chickens.以少获多——一种用于同时检测鸡体内多种甲型流感血凝素类型的定量血清学筛查工具。
PLoS One. 2014 Sep 23;9(9):e108043. doi: 10.1371/journal.pone.0108043. eCollection 2014.
5
Insight into highly conserved H1 subtype-specific epitopes in influenza virus hemagglutinin.深入了解流感病毒血凝素中高度保守的H1亚型特异性表位。
PLoS One. 2014 Feb 26;9(2):e89803. doi: 10.1371/journal.pone.0089803. eCollection 2014.
6
Heterosubtypic immunity to influenza A virus infections in mallards may explain existence of multiple virus subtypes.在野鸭中对甲型流感病毒感染的异型免疫可能解释了多种病毒亚型的存在。
PLoS Pathog. 2013;9(6):e1003443. doi: 10.1371/journal.ppat.1003443. Epub 2013 Jun 20.
7
MDA5 can be exploited as efficacious genetic adjuvant for DNA vaccination against lethal H5N1 influenza virus infection in chickens.MDA5 可作为有效的遗传佐剂，用于针对鸡致命性 H5N1 流感病毒感染的 DNA 疫苗接种。
PLoS One. 2012;7(12):e49952. doi: 10.1371/journal.pone.0049952. Epub 2012 Dec 5.
8
Identification of a highly conserved H1 subtype-specific epitope with diagnostic potential in the hemagglutinin protein of influenza A virus.鉴定甲型流感病毒血凝蛋白中具有潜在诊断价值的高度保守 H1 亚型特异性表位。
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9
Prediction of B-cell linear epitopes with a combination of support vector machine classification and amino acid propensity identification.结合支持向量机分类和氨基酸倾向识别预测B细胞线性表位
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10
Serological characterization of guinea pigs infected with H3N2 human influenza or immunized with hemagglutinin protein.感染 H3N2 人源流感病毒或免疫血凝素蛋白的豚鼠的血清学特征。
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Poult Sci. 2009 Apr;88(4):860-6. doi: 10.3382/ps.2008-00360.
4
Antibody recognition of a highly conserved influenza virus epitope.对高度保守的流感病毒表位的抗体识别。
Science. 2009 Apr 10;324(5924):246-51. doi: 10.1126/science.1171491. Epub 2009 Feb 26.
5
Development of epitope-blocking ELISA for universal detection of antibodies to human H5N1 influenza viruses.用于通用检测人H5N1流感病毒抗体的表位阻断酶联免疫吸附测定法的开发。
PLoS One. 2009;4(2):e4566. doi: 10.1371/journal.pone.0004566. Epub 2009 Feb 24.
6
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Nat Rev Immunol. 2009 Jan;9(1):15-27. doi: 10.1038/nri2454.
7
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8
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J Med Virol. 2008 Nov;80(11):1972-83. doi: 10.1002/jmv.21298.
9
Phylogenetic characterization of H5N1 highly pathogenic avian influenza viruses isolated in Switzerland in 2006.2006年在瑞士分离出的H5N1高致病性禽流感病毒的系统发育特征
Virus Genes. 2008 Dec;37(3):407-13. doi: 10.1007/s11262-008-0285-2. Epub 2008 Sep 12.
10
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J Avian Med Surg. 2008 Mar;22(1):1-16. doi: 10.1647/2006-036R.1.