Post-Graduation Program on Medical Sciences, Fluminense Federal University (UFF), Niteroi, RJ, Brazil.
J Mater Sci Mater Med. 2012 Mar;23(3):781-8. doi: 10.1007/s10856-011-4530-1. Epub 2011 Dec 27.
This work evaluates the suitability of biphasic calcium phosphate (BCP) granules (β-TCP/HA 70:30) as potential carriers for cell-guided bone therapy. The BCP granules were obtained by synthesis in the presence of wax, thermal treatment, crushing and sieving and characterized by scanning electron microscopy (SEM), X-ray diffraction and Fourier transform infrared spectroscopy. The cytocompatibility of the BCP granules was confirmed by a multiparametric cytotoxicity assay. SEM analysis showed human bone cell adhesion and migration after seeding onto the material. Rat subcutaneous xenogeneic grafting of granules associated to human bone cells revealed a more accentuated moderate chronic inflammatory infiltrate, without signs of a strong xenoreactivity. Histomorphometrical analysis of bone repair of defects in rat skulls (∅ = 5 mm) has shown that bone cell associated-BCP and autograft promoted a two- and threefold increase, respectively, on new bone formation after 45 days, as compared to BCP alone and blood clot. The increase in bone repair supports the suitability the biocompatible (70:30) BCP granules as injectable and mouldable scaffolds for human cells in bone bioengineering.
这项工作评估了双相磷酸钙（BCP）颗粒（β-TCP/HA 70:30）作为细胞导向骨治疗潜在载体的适用性。BCP 颗粒通过在存在蜡的情况下合成、热处理、粉碎和筛分获得，并通过扫描电子显微镜（SEM）、X 射线衍射和傅里叶变换红外光谱进行了表征。通过多参数细胞毒性测定证实了 BCP 颗粒的细胞相容性。SEM 分析显示，在将材料接种到细胞上后，人骨细胞的黏附和迁移。与人骨细胞一起将颗粒进行大鼠皮下异种移植，显示出更明显的中度慢性炎症浸润，没有强烈的异种反应迹象。在大鼠颅骨（∅=5mm）缺陷的骨修复的组织形态计量学分析表明，与单独的 BCP 和血凝块相比，与骨细胞相关的 BCP 和自体移植物在 45 天后分别促进了新骨形成增加了两倍和三倍。骨修复的增加支持了生物相容性（70:30）BCP 颗粒作为用于骨生物工程中人类细胞的可注射和可模塑支架的适用性。
