Department of Embryology, Faculty of Biology, Moscow State University, Moscow, Russia.
Dev Dyn. 2010 Mar;239(3):885-96. doi: 10.1002/dvdy.22238.
The mutual arrangement of neural and mesodermal rudiments in artificially bent double explants of Xenopus laevis suprablastoporal areas was compared with that of intact explants. While some of the bent explants straightened or became spherical, most retained and actively reinforced the imposed curvature, creating folds on their concave sides and expanding convex surfaces. In the intact explants, the arrangement of neural and mesodermal rudiments exhibited a distinct antero-posterior polarity, with some variability. In the bent explants, this polarity was lost: the neural rudiments were shifted towards concave while the mesodermal tissues moved towards the convex side, embracing the neural rudiments in a horseshoe-shaped manner. We associate these drastic changes in neuro-mesodermal patterning with the active extension and contraction of the convex and concave sides, respectively, triggered by the imposed deformations. We speculate that similar events are responsible for the establishment of neuro-mesodermal patterns during normal development.
我们比较了人为弯曲的非洲爪蟾原肠顶区双外植体中神经和中胚层原基的相互排列，与完整外植体的排列方式。虽然一些弯曲的外植体变直或变成球形，但大多数外植体保持并积极加强了所施加的曲率，在外植体的凹侧形成褶皱，并使凸面扩张。在完整的外植体中，神经和中胚层原基的排列表现出明显的前后极性，存在一定的可变性。在弯曲的外植体中，这种极性丧失了：神经原基向凹侧移动，而中胚层组织向凸侧移动，以马蹄形的方式包围神经原基。我们将这些神经中胚层模式的剧烈变化与分别由所施加的变形引发的凸侧和凹侧的主动延伸和收缩联系起来。我们推测，在正常发育过程中，类似的事件负责建立神经中胚层模式。
