Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, USA.
J Proteome Res. 2010 May 7;9(5):2182-90. doi: 10.1021/pr900936z.
The rate of tumor recurrence post resection suggests that there are underlying molecular changes in nearby histologically normal tissue that go undetected by conventional diagnostic methods that utilize contrast agents and immunohistochemistry. MALDI MS is a molecular technology that has the specificity and sensitivity to monitor and identify molecular species indicative of these changes. The current study utilizes this technology to assess molecular distributions within a tumor and adjacent normal tissue in clear cell renal cell carcinoma biopsies. Results indicate that the histologically normal tissue adjacent to the tumor expresses many of the molecular characteristics of the tumor. Proteins of the mitochondrial electron transport system are examples of such distributions. This work demonstrates the utility of MALDI MS for the analysis of tumor tissue in the elucidation of aberrant molecular changes in the tumor microenvironment.
切除术后肿瘤复发的比率表明，在常规诊断方法（利用造影剂和免疫组织化学）无法检测到的附近组织中存在潜在的分子变化。MALDI MS 是一种分子技术，具有监测和识别表明这些变化的分子种类的特异性和敏感性。本研究利用该技术评估透明细胞肾细胞癌活检中肿瘤和相邻正常组织内的分子分布。结果表明，肿瘤旁组织在组织学上正常，表达许多肿瘤的分子特征。线粒体电子传递系统的蛋白质就是这种分布的例子。这项工作证明了 MALDI MS 在分析肿瘤组织以阐明肿瘤微环境中异常分子变化方面的应用。
