RNA 病毒、M 卫星、染色体杀伤基因以及 100 株酿酒酵母菌株中的杀伤/非杀伤表型。
RNA viruses, M satellites, chromosomal killer genes, and killer/nonkiller phenotypes in the 100-genomes S. cerevisiae strains.
机构信息
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.
Department of Biology, Duke University, Durham, NC 27708, USA.
出版信息
G3 (Bethesda). 2023 Sep 30;13(10). doi: 10.1093/g3journal/jkad167.
We characterized previously identified RNA viruses (L-A, L-BC, 20S, and 23S), L-A-dependent M satellites (M1, M2, M28, and Mlus), and M satellite-dependent killer phenotypes in the Saccharomyces cerevisiae 100-genomes genetic resource population. L-BC was present in all strains, albeit in 2 distinct levels, L-BChi and L-BClo; the L-BC level is associated with the L-BC genotype. L-BChi, L-A, 20S, 23S, M1, M2, and Mlus (M28 was absent) were in fewer strains than the similarly inherited 2µ plasmid. Novel L-A-dependent phenotypes were identified. Ten M+ strains exhibited M satellite-dependent killing (K+) of at least 1 of the naturally M0 and cured M0 derivatives of the 100-genomes strains; in these M0 strains, sensitivities to K1+, K2+, and K28+ strains varied. Finally, to complement our M satellite-encoded killer toxin analysis, we assembled the chromosomal KHS1 and KHR1 killer genes and used naturally M0 and cured M0 derivatives of the 100-genomes strains to assess and characterize the chromosomal killer phenotypes.
我们对先前鉴定的 RNA 病毒（L-A、L-BC、20S 和 23S）、L-A 依赖性 M 卫星（M1、M2、M28 和 Mlus）以及 M 卫星依赖性杀伤表型在酿酒酵母 100 基因组遗传资源群体中的特征进行了研究。所有菌株中均存在 L-BC，但存在 2 种不同水平，即 L-BChi 和 L-BClo；L-BC 水平与 L-BC 基因型相关。L-BChi、L-A、20S、23S、M1、M2 和 Mlus（M28 不存在）的存在菌株数量少于同样遗传的 2µ 质粒。还鉴定了新的 L-A 依赖性表型。10 株 M+ 菌株表现出至少 1 种天然 M0 和 100 基因组菌株中经 Cure M0 处理的 M0 衍生物的 M 卫星依赖性杀伤（K+）；在这些 M0 菌株中，对 K1+、K2+和 K28+菌株的敏感性有所不同。最后，为了补充我们对 M 卫星编码杀伤毒素的分析，我们组装了染色体 KHS1 和 KHR1 杀伤基因，并使用 100 基因组菌株中的天然 M0 和 Cure M0 衍生物来评估和表征染色体杀伤表型。
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