Institut Pasteur, Unité de Régulation des Infections Rétrovirales, 25 rue du Dr Roux, Paris, France.
J Acquir Immune Defic Syndr. 2010 Apr;53(5):553-63. doi: 10.1097/QAI.0b013e3181cf060f.
Clinical studies support biologically independent roles of cell-free HIV particles and HIV-infected cells in disease progression. The associations between the level of infected cells and immune markers have been poorly studied, particularly in perinatally infected children.
We tested the hypothesis that independent roles of cell-free virus and infected cells in HIV pathogenesis should be revealed by different associations between each of them and specific immune markers.
Levels of HIV RNA and DNA, HIV-specific CD8 T lymphocytes, activated and naive/memory T lymphocytes were determined in 44 untreated HIV-1-infected children. Pearson partial correlation coefficients were used to assess associations between the variables.
Here we provide new information, by showing a direct correlation between the percentages of CD4HLA-DR lymphocytes and HIV DNA levels. Furthermore, higher HIV-specific CD8 T-lymphocyte frequencies were associated with lower HIV DNA levels. In contrast, CD838 lymphocytes and memory CD4 lymphocytes were correlated only to the HIV RNA level. All correlations were independent of age and CD4 depletion.
Several immune markers were correlated to either the HIV RNA or the HIV DNA level, but never to both of them, supporting the concept that cell-free virus and infected cells play different roles in HIV-1 immunopathogenesis.
临床研究支持游离 HIV 颗粒和感染 HIV 的细胞在疾病进展中具有生物学上的独立性作用。然而，关于感染细胞水平与免疫标志物之间的关联研究甚少，尤其是在围生期感染的儿童中。
我们检验了一个假设，即游离病毒和感染细胞在 HIV 发病机制中的独立作用，应该通过它们与特定免疫标志物之间的不同关联来揭示。
我们测定了 44 例未经治疗的 HIV-1 感染儿童的游离 HIV RNA 和 DNA 水平、HIV 特异性 CD8 T 淋巴细胞、活化和幼稚/记忆 T 淋巴细胞。采用 Pearson 偏相关系数评估各变量之间的关联。
在此，我们提供了新的信息，表明 CD4 HLA-DR 淋巴细胞的百分比与 HIV DNA 水平呈直接相关。此外，HIV 特异性 CD8 T 淋巴细胞的频率越高，HIV DNA 水平越低。相比之下，CD838 淋巴细胞和记忆性 CD4 淋巴细胞仅与 HIV RNA 水平相关。所有的相关性均独立于年龄和 CD4 细胞耗竭。
多个免疫标志物与 HIV RNA 或 HIV DNA 水平相关，但从不与两者都相关，这支持了游离病毒和感染细胞在 HIV-1 免疫发病机制中发挥不同作用的概念。
