Department of Petroleum Microbiology, Institute of Biotechnology, VAST, Hoang Quoc Viet-Cau Giay-Hanoi-Vietnam.
J Basic Microbiol. 2010 Jun;50(3):241-53. doi: 10.1002/jobm.200900358.
A soil bacterium isolated from oil-polluted sand samples collected in the Saudi Arabian Desert has been determined as Nocardia cyriacigeorgica, which has a high capacity of degrading and utilizing a broad range of hydrocarbons. The metabolic pathways of three classes of hydrocarbons were elucidated by identifying metabolites in cell-free extracts analyzed by GC/MS and HPLC/UV-Vis in comparison with standard compounds. During tetradecane oxidation, tetradecanol; tetradecanoic acid; dodecanoic acid; decanoic acid could be found as metabolites, indicating a monoterminal degradation pathway of n -alkanes. The oxidation of pristane resulted in the presence of pristanoic acid; 2-methylglutaric acid; 4,8-dimethylnonanoic acid; and 2,6-dimethylheptanoic acid, which give rise to a possible mono- and di-terminal oxidation. In case of sec -octylbenzene, eight metabolites were detected including 5-phenylhexanoic acid; 3-phenylbutyric acid; 2-phenylpropionic acid; beta -methylcinnamic acid; acetophenone; beta -hydroxy acetophenone; 2,3-dihydroxy benzoic acid and succinic acid. From these intermediates a new degradation pathway for sec -octylbenzene was investigated. Our results indicate that N. cyriacigeorgica has the ability to degrade aliphatic and branched chain alkanes as well as alkylbenzene effectively and, therefore, N. cyriacigeorgica is probably a suitable bacterium for biodegradation of oil or petroleum products in contaminated soils.
从沙特阿拉伯沙漠中采集的含油污染沙样中分离到的一种土壤细菌被确定为触酶糖丝菌，它具有很强的降解和利用各种烃类的能力。通过比较 GC/MS 和 HPLC/UV-Vis 分析的无细胞提取物中的代谢产物与标准化合物，阐明了三类烃的代谢途径。在十四烷氧化过程中，可以发现十四烷醇；十四烷酸；十二烷酸；癸酸作为代谢物，表明 n-烷烃具有单末端降解途径。姥鲛烷的氧化导致存在姥鲛酸；2-甲基戊二酸；4,8-二甲基壬酸；和 2,6-二甲基庚酸，这可能导致单末端和双末端氧化。对于仲辛基苯，检测到 8 种代谢产物，包括 5-苯基己酸；3-苯基丁酸；2-苯基丙酸；β-甲基肉桂酸；苯乙酮；β-羟基苯乙酮；2,3-二羟基苯甲酸和琥珀酸。从这些中间产物中，研究了仲辛基苯的新降解途径。研究结果表明，触酶糖丝菌具有有效降解脂肪族和支链烷烃以及烷基苯的能力，因此，触酶糖丝菌可能是生物降解污染土壤中石油或石油产品的合适细菌。
