Marine Microbiology, Faculty of Bioresources, Mie University, Kurimamachiya-cho, Tsu, Mie, Japan.
J Basic Microbiol. 2010 Jun;50(3):285-9. doi: 10.1002/jobm.200900250.
Marinobacter comprises Gram-negative, aerobic, motile, and rod-shaped bacteria within the gamma-subclass of the Proteobacteria and is known to be halophilic or halotolerant, heterotrophic neutrophile. Two strains classified as belonging to Marinobacter, named PAD-2 and SeT-1, were isolated from marine sediment. The most closely related species of PAD-2 and SeT-1 are M. alkaliphilus and M. guinea, respectively. The strain PAD-2 exhibited remarkably higher denitrification at concentrations of 0.5 to 1 M NaCl (3-6% w/w) than at other salinities (2 and 3 M NaCl, 12-18% w/w), and optimal denitrification was observed in media with 0.5 M NaCl. The effect of pH on denitrification by strain PAD-2 was also examined, and the optimum denitrification occurred at neutral pH rather than under alkaline conditions. Overall, strain PAD-2 appears to be a novel halotolerant species belonging to the genus Marinobacter that shares many characteristics, such as substrate utilization profile and optimum NaCl concentration for growth with M. alkaliphilus.
海洋盐单胞菌属于γ-变形菌纲，是革兰氏阴性、需氧、能运动的杆状细菌，为嗜盐或耐盐的异养兼性厌氧菌。从海洋沉积物中分离到的两种海洋盐单胞菌（命名为 PAD-2 和 SeT-1）属于海洋盐单胞菌。与 PAD-2 和 SeT-1 最密切相关的种分别是 M. alkaliphilus 和 M. guinea。与其他盐度（2 和 3 M NaCl，12-18% w/w）相比，菌株 PAD-2 在 0.5 至 1 M NaCl（3-6% w/w）浓度下的脱氮能力显著提高，在 0.5 M NaCl 培养基中观察到最佳脱氮效果。还研究了 pH 值对 PAD-2 菌株脱氮的影响，最佳脱氮发生在中性 pH 值而不是碱性条件下。总的来说，菌株 PAD-2 似乎是一种新型的耐盐种，属于海洋盐单胞菌属，与 M. alkaliphilus 具有许多相似的特征，如底物利用谱和生长的最佳 NaCl 浓度。
