Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore, Singapore.
J Chromatogr A. 2010 Mar 12;1217(11):1862-6. doi: 10.1016/j.chroma.2010.01.065. Epub 2010 Jan 25.
Microfluidic devices are capable of separating microparticles and cells. We developed and tested the efficiency of silicon cross-flow microfilters for the separation of primitive fetal nucleated red blood cells (FNRBCs) and adult anucleate red blood cell (AARBCs) from model mixtures. Stepwise improvements over three generations of device design resulted in an increasing trend in the recovery of FNRBCs. We obtained a recovery of FNRBCs (74.0+/-6.3%, p<0.05, n=5) using the third generation device, with a depletion of 46.5+/-3.2% AARBCs from the cell mixture. The purity of FNRBCs in the enriched fraction was enhanced by a factor of 1.7-fold.
微流控装置能够分离微颗粒和细胞。我们开发并测试了硅横流微滤器从模型混合物中分离原始胎儿有核红细胞（FNRBC）和成人无核红细胞（AARBC）的效率。经过三代设备设计的逐步改进，FNRBC 的回收率呈上升趋势。我们使用第三代设备获得了 FNRBC 的回收率（74.0+/-6.3%，p<0.05，n=5），同时从细胞混合物中去除了 46.5+/-3.2%的 AARBC。富集部分中 FNRBC 的纯度提高了 1.7 倍。
