F344/BN 大鼠基底节和皮质中 HSP25 表达的年龄相关性变化。
Age-related changes in HSP25 expression in basal ganglia and cortex of F344/BN rats.
机构信息
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS 66160, United States.
出版信息
Neurosci Lett. 2010 Mar 19;472(2):90-3. doi: 10.1016/j.neulet.2010.01.049. Epub 2010 Feb 6.
Abstract
Normal aging is associated with chronic oxidative stress. In the basal ganglia, oxidative stress may contribute to the increased risk of Parkinson's disease in the elderly. Neurons are thought to actively utilize compensatory defense mechanisms, such as heat shock proteins (HSPs), to protect from persisting stress. Despite their protective role, little is known about HSP expression in the aging basal ganglia. The purpose of this study was to examine HSP expression in striatum, substantia nigra, globus pallidus and cortex in 6-, 18- and 30-month-old Fischer 344/Brown Norway rats. We found robust age-related increases in phosphorylated and total HSP25 in each brain region studied. Conversely, HSP72 (the inducible form of HSP70) was reduced with age, but only in the striatum. p38 MAPK, a protein implicated in activating HSP25, did not change with age, nor did HSC70 (the constitutive form of HSP70), or HSP60. These results suggest that HSP25 is especially responsive to age-related stress in the basal ganglia.
摘要
正常衰老与慢性氧化应激有关。在基底神经节中，氧化应激可能导致老年人帕金森病风险增加。人们认为神经元会主动利用热休克蛋白（HSPs）等补偿性防御机制来抵御持续的应激。尽管它们具有保护作用，但对于衰老基底神经节中的 HSP 表达知之甚少。本研究旨在检查 6、18 和 30 月龄 Fischer 344/布朗挪威大鼠纹状体、黑质、苍白球和皮质中的 HSP 表达。我们发现，在研究的每个脑区，磷酸化和总 HSP25 都与年龄呈强相关增加。相反，HSP72（HSP70 的诱导形式）随年龄减少，但仅在纹状体中。p38 MAPK 是一种与 HSP25 激活有关的蛋白，其随年龄变化不大，HSC70（HSP70 的组成型形式）或 HSP60 也没有变化。这些结果表明，HSP25 对基底神经节中与年龄相关的应激特别敏感。
相似文献
1
Age-related changes in HSP25 expression in basal ganglia and cortex of F344/BN rats.F344/BN 大鼠基底节和皮质中 HSP25 表达的年龄相关性变化。
Neurosci Lett. 2010 Mar 19;472(2):90-3. doi: 10.1016/j.neulet.2010.01.049. Epub 2010 Feb 6.
2
Heat shock proteins HSP25, HSP60, HSP72, HSP73 in isoosmotic cortex and hyperosmotic medulla of rat kidney.大鼠肾脏等渗皮质和高渗髓质中的热休克蛋白HSP25、HSP60、HSP72、HSP73
Pflugers Arch. 1996 Feb;431(4):608-17. doi: 10.1007/BF02191910.
3
Effects of long-term changes in medullary osmolality on heat shock proteins HSp25, HSP60, HSP72 and HSP73 in the rat kidney.髓质渗透压长期变化对大鼠肾脏热休克蛋白HSp25、HSP60、HSP72和HSP73的影响。
Pflugers Arch. 1998 Apr;435(5):705-12. doi: 10.1007/s004240050572.
4
Resistance training increases heat shock protein levels in skeletal muscle of young and old rats.阻力训练可提高年轻和老年大鼠骨骼肌中的热休克蛋白水平。
Exp Gerontol. 2006 Apr;41(4):398-406. doi: 10.1016/j.exger.2006.01.005. Epub 2006 Mar 9.
5
Phosphorylation-dependent cellular localization and thermoprotective role of heat shock protein 25 in hippocampal progenitor cells.热休克蛋白25在海马祖细胞中的磷酸化依赖性细胞定位及热保护作用
J Biol Chem. 2002 May 31;277(22):19913-21. doi: 10.1074/jbc.M104396200. Epub 2002 Mar 22.
6
Age-related differences in skeletal muscle insulin signaling: the role of stress kinases and heat shock proteins.骨骼肌胰岛素信号传导中的年龄相关差异：应激激酶和热休克蛋白的作用。
J Appl Physiol (1985). 2008 Sep;105(3):839-48. doi: 10.1152/japplphysiol.00148.2008. Epub 2008 Jul 3.
7
Measures of striatal insulin resistance in a 6-hydroxydopamine model of Parkinson's disease.帕金森病6-羟基多巴胺模型中纹状体胰岛素抵抗的测量
Brain Res. 2008 Nov 13;1240:185-95. doi: 10.1016/j.brainres.2008.08.089. Epub 2008 Sep 11.
8
Changes in mRNA levels for heat-shock/stress proteins (Hsp) and a secretory vesicle associated cysteine-string protein (Csp1) after amphetamine (AMPH) exposure.苯丙胺（AMPH）暴露后热休克/应激蛋白（Hsp）和一种与分泌囊泡相关的半胱氨酸串珠蛋白（Csp1）的mRNA水平变化。
Ann N Y Acad Sci. 1999;890:314-29. doi: 10.1111/j.1749-6632.1999.tb08009.x.
9
Stimulation of multiple mitogen-activated protein kinase sub-families by oxidative stress and phosphorylation of the small heat shock protein, HSP25/27, in neonatal ventricular myocytes.氧化应激对新生大鼠心室肌细胞中多个丝裂原活化蛋白激酶亚家族的刺激作用以及小热休克蛋白HSP25/27的磷酸化
Biochem J. 1998 Aug 1;333 ( Pt 3)(Pt 3):581-9. doi: 10.1042/bj3330581.
10
Contribution of IL-6 to the Hsp72, Hsp25, and alphaB-crystallin [corrected] responses to inflammation and exercise training in mouse skeletal and cardiac muscle.白细胞介素-6对小鼠骨骼肌和心肌中热休克蛋白72、热休克蛋白25及αB-晶状体蛋白[校正后]对炎症和运动训练反应的作用。
J Appl Physiol (1985). 2008 Dec;105(6):1830-6. doi: 10.1152/japplphysiol.90955.2008. Epub 2008 Oct 16.
引用本文的文献
1
Role of heat shock proteins in aging and chronic inflammatory diseases.热休克蛋白在衰老和慢性炎症性疾病中的作用。
Geroscience. 2021 Oct;43(5):2515-2532. doi: 10.1007/s11357-021-00394-2. Epub 2021 Jul 9.
2
Molecular Chaperones and Proteolytic Machineries Regulate Protein Homeostasis In Aging Cells.分子伴侣和蛋白水解机器调节衰老细胞中的蛋白质平衡。
Cells. 2020 May 24;9(5):1308. doi: 10.3390/cells9051308.
3
Heat-Shock Proteins in Neuroinflammation.神经炎症中的热休克蛋白
Front Pharmacol. 2019 Aug 27;10:920. doi: 10.3389/fphar.2019.00920. eCollection 2019.
4
Heat shock protein defenses in the neocortex and allocortex of the telencephalon.端脑新皮层和旧皮层中的热休克蛋白防御机制。
Neurobiol Aging. 2015 May;36(5):1924-37. doi: 10.1016/j.neurobiolaging.2015.02.011. Epub 2015 Feb 18.
5
Heat shock protein responses to aging and proteotoxicity in the olfactory bulb.嗅球中热休克蛋白对衰老和蛋白毒性的反应。
J Neurochem. 2015 Jun;133(6):780-794. doi: 10.1111/jnc.13041. Epub 2015 Mar 5.
6
Heat shock proteins in neurodegenerative disorders and aging.神经退行性疾病和衰老中的热休克蛋白
J Cell Commun Signal. 2014 Dec;8(4):293-310. doi: 10.1007/s12079-014-0243-9. Epub 2014 Sep 11.
7
Adaptation and sensitization to proteotoxic stress.适应和敏化蛋白质毒性应激。
Dose Response. 2013 Aug 5;12(1):24-56. doi: 10.2203/dose-response.13-016.Leak. eCollection 2014 Jan.
8
The expression and release of Hsp60 in 6-OHDA induced in vivo and in vitro models of Parkinson's disease.帕金森病体内和体外模型中 6-OHDA 诱导的 Hsp60 的表达和释放。
Neurochem Res. 2013 Oct;38(10):2180-9. doi: 10.1007/s11064-013-1127-8. Epub 2013 Aug 14.
9
Basal and stress-induced Hsp70 are modulated by ataxin-3.ataxin-3 调节基础状态和应激诱导的 Hsp70。
Cell Stress Chaperones. 2012 Nov;17(6):729-42. doi: 10.1007/s12192-012-0346-2. Epub 2012 Jul 10.
本文引用的文献
1
Measures of striatal insulin resistance in a 6-hydroxydopamine model of Parkinson's disease.帕金森病6-羟基多巴胺模型中纹状体胰岛素抵抗的测量
Brain Res. 2008 Nov 13;1240:185-95. doi: 10.1016/j.brainres.2008.08.089. Epub 2008 Sep 11.
2
Age-related differences in skeletal muscle insulin signaling: the role of stress kinases and heat shock proteins.骨骼肌胰岛素信号传导中的年龄相关差异：应激激酶和热休克蛋白的作用。
J Appl Physiol (1985). 2008 Sep;105(3):839-48. doi: 10.1152/japplphysiol.00148.2008. Epub 2008 Jul 3.
3
Mitochondria and neurodegeneration.线粒体与神经退行性变
Novartis Found Symp. 2007;287:183-92; discussion 192-6. doi: 10.1002/9780470725207.ch13.
4
Age-related changes in orolingual motor function in F344 vs F344/BN rats.F344 大鼠与 F344/BN 大鼠口面部运动功能的年龄相关变化
Physiol Behav. 2008 Feb 27;93(3):461-6. doi: 10.1016/j.physbeh.2007.10.004. Epub 2007 Oct 12.
5
Heat shock proteins and protection of the nervous system.热休克蛋白与神经系统的保护
Ann N Y Acad Sci. 2007 Oct;1113:147-58. doi: 10.1196/annals.1391.032. Epub 2007 Jul 26.
6
PKD, PKD2, and p38 MAPK mediate Hsp27 serine-82 phosphorylation induced by neurotensin in pancreatic cancer PANC-1 cells.多囊肾病蛋白1（PKD）、多囊肾病蛋白2（PKD2）和p38丝裂原活化蛋白激酶（p38 MAPK）介导神经降压素在胰腺癌PANC-1细胞中诱导的热休克蛋白27（Hsp27）丝氨酸82位点磷酸化。
J Cell Biochem. 2008 Feb 1;103(2):648-62. doi: 10.1002/jcb.21439.
7
Measuring forelimb force control and movement in Fischer 344/Brown Norway rats: effects of age and lorazepam.测量Fischer 344/布朗挪威大鼠的前肢力量控制和运动：年龄和劳拉西泮的影响。
Behav Pharmacol. 2006 Dec;17(8):725-30. doi: 10.1097/FBP.0b013e32801155e8.
8
Effects of aging and anti-aging caloric restrictions on carbonyl and heat shock protein levels and expression.衰老及抗衰老热量限制对羰基和热休克蛋白水平及表达的影响。
Biogerontology. 2005 Dec;6(6):397-406. doi: 10.1007/s10522-005-4906-z.
9
Bilateral effects of unilateral intrastriatal GDNF on locomotor-excited and nonlocomotor-related striatal neurons in aged F344 rats.单侧纹状体内注射胶质细胞源性神经营养因子对老年F344大鼠运动兴奋型和非运动相关纹状体神经元的双侧影响
Neurobiol Aging. 2007 Jan;28(1):156-65. doi: 10.1016/j.neurobiolaging.2005.10.015. Epub 2005 Nov 28.
10
Hsp27 inhibits 6-hydroxydopamine-induced cytochrome c release and apoptosis in PC12 cells.热休克蛋白27抑制6-羟基多巴胺诱导的PC12细胞细胞色素c释放和凋亡。
Biochem Biophys Res Commun. 2005 Feb 18;327(3):801-10. doi: 10.1016/j.bbrc.2004.12.066.
Zotero 插件