哺乳动物自噬研究方法。
Methods in mammalian autophagy research.
机构信息
Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113-8519, Japan.
出版信息
Cell. 2010 Feb 5;140(3):313-26. doi: 10.1016/j.cell.2010.01.028.
Abstract
Autophagy has been implicated in many physiological and pathological processes. Accordingly, there is a growing scientific need to accurately identify, quantify, and manipulate the process of autophagy. However, as autophagy involves dynamic and complicated processes, it is often analyzed incorrectly. In this Primer, we discuss methods to monitor autophagy and to modulate autophagic activity, with a primary focus on mammalian macroautophagy.
摘要
自噬作用涉及许多生理和病理过程。因此，科学界越来越需要准确地识别、定量和操纵自噬过程。然而，由于自噬涉及动态和复杂的过程，因此经常会被错误地分析。在这篇概述中，我们讨论了监测自噬和调节自噬活性的方法，主要侧重于哺乳动物巨自噬。
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