Division of Molecular Biology, Department of Zoology, Sri Venkateswara University, Tirupati, India.
Epilepsy Behav. 2010 Mar;17(3):332-5. doi: 10.1016/j.yebeh.2010.01.002. Epub 2010 Feb 9.
The study described here was carried out to investigate the anticonvulsant effect of different extracts of Centella asiatica with respect to cholinergic activity on pentylenetetrazol (PTZ)-induced seizures. Rats were randomly divided into eight groups of six rats each: nonepileptic rats treated with saline; PTZ (60 mg/kg, IP)-induced seizure rats treated with saline; PTZ-induced seizure rats pretreated with n-hexane, chloroform, ethyl acetate, n-butanol, and water extracts of C. asiatica; and PTZ-induced seizure rats pretreated with diazepam (2mg/kg body wt). The seized rats pretreated with different extracts were administered a dose of 200mg/kg body wt orally for 1 week before induction of epilepsy. Increased acetylcholine content and decreased acetylcholinesterase activity were recorded in different brain regions during PTZ-induced seizures. Pretreatment with C. asiatica extracts caused recovery of the levels of acetylcholine and acetylcholinesterase. These findings suggest that C. asiatica causes perceptible changes in the cholinergic system as one of the facets of its anticonvulsant activity.
本研究旨在探讨积雪草不同提取物对戊四氮（PTZ）诱导的癫痫发作的抗惊厥作用及其对胆碱能活性的影响。大鼠随机分为八组，每组六只：生理盐水处理的非癫痫大鼠；生理盐水处理的 PTZ（60mg/kg，IP）诱导的癫痫发作大鼠；用正己烷、氯仿、乙酸乙酯、正丁醇和水提取物预处理的 PTZ 诱导的癫痫发作大鼠；用安定（2mg/kg 体重）预处理的 PTZ 诱导的癫痫发作大鼠。不同提取物预处理的癫痫发作大鼠在癫痫发作诱导前口服 200mg/kg 体重，每周一次，共 1 周。在 PTZ 诱导的癫痫发作期间，不同脑区的乙酰胆碱含量增加，乙酰胆碱酯酶活性降低。积雪草提取物预处理可恢复乙酰胆碱和乙酰胆碱酯酶的水平。这些发现表明，积雪草作为其抗惊厥作用的一个方面，可引起胆碱能系统的明显变化。
