Department of Otolaryngology and Head and Neck Surgery, Helsinki University Central Hospital, Haartmaninkatu 4E, Helsinki, Finland.
Br J Cancer. 2010 Mar 2;102(5):892-7. doi: 10.1038/sj.bjc.6605544. Epub 2010 Feb 9.
The prognosis of squamous cell carcinoma of the oral tongue is poor and it would be beneficial to find prognostic markers to better adjust treatment. Bmi-1 controls cell cycle and self-renewal of tissue stem cells, transcription factor c-myc affects cell proliferation and apoptosis, and Snail regulates epithelial-mesenchymal transition. The expression of these markers has been connected to prognosis in many cancer types.
Bmi-1, c-myc, and Snail expressions were studied in our material consisting of 73 primarily T1N0M0 oral tongue carcinoma patients. We compared the immunoexpressions of Bmi-1, c-myc, and Snail with clinical parameters including the degree of histological differentiation, tumour size, TNM classification, depth of invasion, and resection margins. In addition, survival analyses were performed, comparing disease-free survival time with the registered protein expression of the markers mentioned above.
A significant correlation between Bmi-1 protein expression and recurrence (log-rank test, P=0.005) was detected. Snail and c-myc expression did not correlate with prognosis. Snail expression correlated with histopathological grade (Fisher's exact test, P=0.007) and with the invasion depth of tumours (chi(2)-test, P=0.037).
Negative Bmi-1 immunoexpression might serve as a marker of poor prognosis in oral tongue carcinoma patients.
口腔舌鳞状细胞癌的预后较差，因此找到预后标志物来更好地调整治疗方法将是有益的。Bmi-1 控制组织干细胞的细胞周期和自我更新，转录因子 c-myc 影响细胞增殖和凋亡，而 Snail 调节上皮-间充质转化。这些标志物的表达已与许多癌症类型的预后相关。
我们的研究材料包括 73 例原发性 T1N0M0 口腔舌癌患者，研究了 Bmi-1、c-myc 和 Snail 的表达情况。我们将 Bmi-1、c-myc 和 Snail 的免疫表达与临床参数（包括组织学分化程度、肿瘤大小、TNM 分类、浸润深度和切除边缘）进行了比较。此外，还进行了生存分析，将无病生存时间与上述标志物的登记蛋白表达进行了比较。
Bmi-1 蛋白表达与复发之间存在显著相关性（对数秩检验，P=0.005）。Snail 和 c-myc 表达与预后无关。Snail 表达与组织病理学分级（Fisher 确切检验，P=0.007）和肿瘤浸润深度（卡方检验，P=0.037）相关。
Bmi-1 免疫表达阴性可能是口腔舌癌患者预后不良的标志物。
