Nie Q, Fang M, Xie L, Peng X, Xu H, Luo C, Zhang D, Zhang X
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
J Biomed Biotechnol. 2009;2009:567120. doi: 10.1155/2009/567120. Epub 2010 Jan 20.
Ghrelin (GHRL) and its receptor (GHSR) are involved in various bioactivities. In this study, the complete cDNA and 5' flanking region of the duck GHRL (dGHRL) gene and a 3717 bp fragment of the duck GHSR (dGHSR) gene were obtained. A total of 19, 8, 43, and 48 SNPs identified in 2751, 1358, 3671, and 3567 bp of the chicken GHRL (cGHRL), chicken GHSR (cGHSR), dGHRL, and dGHSR genes, respectively. Both cGHRL and dGHRL were expressed predominantly in the proventriculus, whereas the highest mRNA levels of cGHSR and dGHSR were detected in the breast muscle and pituitary. Association analysis showed that C-2047G, A-2355C, and A-2220C of the cGHRL gene were significantly associated with abdominal fat weight (AFW; P = .01), crude protein content of leg muscle (CPCLM; P = .02), and CPCLM (P = .0009), respectively. C-1459T of the cGHSR gene was also significantly associated with CPCLM (P = .0004). C-729T of dGHRL and A3427T of dGHSR were both significantly associated with subcutaneous fat thickness (SFT; P = .04). It was indicated by this study that the GHRL and GHSR genes were related to fat deposition in both chicken and duck.
胃饥饿素（GHRL）及其受体（GHSR）参与多种生物活性。在本研究中，获得了鸭GHRL（dGHRL）基因的完整cDNA和5'侧翼区域以及鸭GHSR（dGHSR）基因的一个3717 bp片段。分别在鸡GHRL（cGHRL）、鸡GHSR（cGHSR）、dGHRL和dGHSR基因的2751、1358、3671和3567 bp中鉴定出19、8、43和48个单核苷酸多态性（SNP）。cGHRL和dGHRL均主要在前胃中表达，而cGHSR和dGHSR的最高mRNA水平在胸肌和垂体中检测到。关联分析表明，cGHRL基因的C-2047G、A-2355C和A-2220C分别与腹脂重（AFW；P = 0.01）、腿部肌肉粗蛋白含量（CPCLM；P = 0.02）和CPCLM（P = 0.0009）显著相关。cGHSR基因的C-1459T也与CPCLM显著相关（P = 0.0004）。dGHRL的C-729T和dGHSR的A3427T均与皮下脂肪厚度（SFT；P = 0.04）显著相关。本研究表明，GHRL和GHSR基因与鸡和鸭的脂肪沉积有关。
