Laboratorio de Inmunología y Virología, Departamento de Microbiología e Inmunología, Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Mexico.
J Nanobiotechnology. 2010 Jan 20;8:1. doi: 10.1186/1477-3155-8-1.
Silver nanoparticles have proven to exert antiviral activity against HIV-1 at non-cytotoxic concentrations, but the mechanism underlying their HIV-inhibitory activity has not been not fully elucidated. In this study, silver nanoparticles are evaluated to elucidate their mode of antiviral action against HIV-1 using a panel of different in vitro assays.
Our data suggest that silver nanoparticles exert anti-HIV activity at an early stage of viral replication, most likely as a virucidal agent or as an inhibitor of viral entry. Silver nanoparticles bind to gp120 in a manner that prevents CD4-dependent virion binding, fusion, and infectivity, acting as an effective virucidal agent against cell-free virus (laboratory strains, clinical isolates, T and M tropic strains, and resistant strains) and cell-associated virus. Besides, silver nanoparticles inhibit post-entry stages of the HIV-1 life cycle.
These properties make them a broad-spectrum agent not prone to inducing resistance that could be used preventively against a wide variety of circulating HIV-1 strains.
银纳米粒子已被证明在非细胞毒性浓度下对 HIV-1 具有抗病毒活性，但它们抑制 HIV 的活性机制尚未完全阐明。在这项研究中，我们使用一系列不同的体外检测方法来评估银纳米粒子对 HIV-1 的抗病毒作用方式。
我们的数据表明，银纳米粒子在病毒复制的早期发挥抗 HIV 活性，很可能作为一种病毒杀灭剂或病毒进入抑制剂。银纳米粒子以一种阻止 CD4 依赖性病毒结合、融合和感染性的方式结合 gp120，对无细胞病毒（实验室株、临床分离株、T 和 M 嗜性株以及耐药株）和细胞相关病毒具有有效的病毒杀灭作用。此外，银纳米粒子抑制 HIV-1 生命周期的进入后阶段。
这些特性使它们成为一种不易产生耐药性的广谱药物，可用于预防广泛传播的 HIV-1 株。
