Mlinarić Nives Matijaković, Zore Anamarija, Veselinovic Valentina, Trtić Nataša, Dolić Olivera, Štukelj Roman, Abram Anže, Učakar Aleksander, Adamović Tijana, Vidrih Rajko, Rojko Franc, Kasemets Kaja, Kahru Anne, Bohinc Klemen
Faculty of Health Sciences, University of Ljubljana, Zdravstvena pot, 1000 Ljubljana, Slovenia.
Faculty of Medicine, University of Banja Luka, 78000 Banja Luka, Bosnia and Herzegovina.
ACS Omega. 2025 Mar 28;10(13):13060-13072. doi: 10.1021/acsomega.4c10170. eCollection 2025 Apr 8.
Oral health represents a significant factor in general health and life quality. A significant number of people are affected by tooth loss during their lifetimes, especially in the older population. Poly(methyl methacrylate) (PMMA) resins are the preferred option for replacing missing teeth due to the material stability, easy handling, low toxicity, and most importantly biocompatibility with human tissue. Even though PMMA is the preferable material for denture preparation, it is susceptible to microbial colonization, which can induce the development of oral infections. This study aimed to increase the antimicrobial effect of PMMA and compare the antimicrobial properties of PMMA incorporated with different amounts (2 and 5 wt %) of zinc oxide (ZnO; primary size 62.4 nm ± 16.7 nm) and copper oxide (CuO; primary size 434.0 nm ± 118.5 nm) nanoparticles to determine their antimicrobial effects on Gram-positive bacteria and yeast -pathogenic microbes often found on dentures. To understand the adhesion of microorganisms to PMMA-modified surfaces, the following surface properties were measured: roughness, contact angle, and ζ potential. In addition, CIE (the International Commission on Illumination) color parameters of the materials were determined. The bacterial adhesion was measured by viable plate counts and scanning electron microscopy. Our study showed that 5 wt % ZnO added to PMMA yields a promising denture material that is esthetically acceptable and shows antimicrobial properties toward both, and .
口腔健康是整体健康和生活质量的一个重要因素。相当多的人在其一生中会受到牙齿缺失的影响，尤其是老年人群体。聚甲基丙烯酸甲酯（PMMA）树脂由于材料稳定性、易于操作、低毒性，以及最重要的与人体组织的生物相容性，是替代缺失牙齿的首选材料。尽管PMMA是义齿制备的首选材料，但它易受微生物定植影响，这会引发口腔感染。本研究旨在增强PMMA的抗菌效果，并比较掺入不同量（2重量%和5重量%）氧化锌（ZnO；初级粒径62.4纳米±16.7纳米）和氧化铜（CuO；初级粒径434.0纳米±118.5纳米）纳米颗粒的PMMA的抗菌性能，以确定它们对革兰氏阳性菌和酵母——义齿上常见的致病微生物的抗菌效果。为了解微生物对PMMA改性表面的粘附情况，测量了以下表面性质：粗糙度、接触角和ζ电位。此外，还测定了材料的CIE（国际照明委员会）颜色参数。通过活菌平板计数和扫描电子显微镜测量细菌粘附情况。我们的研究表明，向PMMA中添加5重量%的ZnO可得到一种有前景的义齿材料，其在美学上是可接受的，并且对革兰氏阳性菌和酵母均显示出抗菌性能。
